@article{Porras-Vega_Váldez-Núñez_Gómez-Pérez_Ruiz-Ramirez_Romero-Batista_2021, title={Circular dichroism and fluorescence in the evaluation of the chemical-physical and biological reference material of nimotuzumab}, volume={33}, url={https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5164}, abstractNote={<p>The establishment of a reference material is necessary for the quality control tests that are<br>carried out on drugs, guaranteeing reliable and traceable measurements. A new candidate<br>reference material for Nimotuzumab was developed at the Center for Molecular<br>Immunology with the aim of replacing the current one before its expiration. In this work,<br>was determined the secondary structure and thermal stability of materials by circular<br>dichroism in the ultraviolet distant and three-dimensional folding by means of the near<br>ultraviolet and intrinsic fluorescence. Both materials presented a predominance of β sheets<br>(~ 42%) and thermodynamic stability of ~ 71 o C. The three-dimensional folding showed<br>very similar spectra in terms of the position of the absorption bands of the aromatic<br>residues and the intensity of fluorescence emission for the Tyr (336 nm) and for the Trp<br>(337 nm).</p>}, number={1}, journal={Revista Cubana de Química}, author={Porras-Vega, Stephania and Váldez-Núñez, Roxana and Gómez-Pérez, Jose Arberto and Ruiz-Ramirez, Ingrid and Romero-Batista, Yamilet de la Rosa}, year={2021}, month={Mar.}, pages={3–21} }