Characterization of osmotic fragility of human erythrocytes in chronic haemolytic anemia

Authors

  • MSc. Yamirka Alonso-Geli Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba
  • MSc. Yamisleydi Alonso-Moreno Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba
  • Lic. José E. Falcón-Diéguez Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba
  • Est. Liset Lucambio-Miró Centro de Biofísica Médica, Universidad de Oriente, Santiago de Cuba

Abstract

Since sickle cell diseases  are  characterized by chronic hemolyticanemia,  this work aims to characterize the osmotic fragility of normal and sickled erythrocytes.  We worked with blood samples of healthy individuals and sickle cell patients. We carry out the rapid  osmotic fragility test. The results show that the minimum globular resistance was   at  0,45  % NaCl and the maximum resistance was at 0,32 % NaCl. In sickle cell, resistance was at 0,36  % NaCl and the maximum globular resistance was at 0,1 % NaCl. By comparing the results for each type of cell, we obtain decrease of osmotic fragility of the sickle cells against normal cells, showing a shift of the curve  towards lower values of  % NaCl. We found statistically significant differences between the means of the percentage of hemolysis in hypotonic solutions between 0,1 % and 0,36 % NaCl (p < 0,05).

Keywords: osmotic fragility, erythrocytes, sickle cells, sickle cell anemia.

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Published

2015-10-30

How to Cite

Alonso-Geli, M. Y., Alonso-Moreno, M. Y., Falcón-Diéguez, L. J. E., & Lucambio-Miró, E. L. (2015). Characterization of osmotic fragility of human erythrocytes in chronic haemolytic anemia. Revista Cubana De Química, 27(2), 110–118. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/250

Issue

Vol. 27 No. 2 (2015)

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Artículos

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