Production of Gp-41 (HIV-1) recombinant protein in E.coli: effect of magnetic field

Authors

  • Dr.C. Ramón Emilio Narciandi-Díaz Centro de Ingeniería Genética y Biotecnología, Ciudad Habana, Cuba
  • MSc. Juan Miguel Rivera-Martin Centro de Ingeniería Genética y Biotecnología, Ciudad Habana, Cuba

Keywords:

E. coli, Gp-41, HIV-1, fed batch culture, magnetic field

Abstract

The influence of the extremely low frequency electromagnetic field was studied in a fed batch culture of Escherichia coli (E.coli), developed for the production of the recombinant Gp-41 protein of human immunodeficiency virus type 1 (HIV-1). An electromagnet, which generates non-homogeneous magnetic fields, was used, at a constant induction value of 0.055 Tesla, and exposure time of 6, 5 h. With the application of the magnetic field, a 1, 2-fold increase in volumetric production (320 mg / L) and 1,5 times in process productivity (30 mg / L) were obtained. At the same time, acetate production decreased 20 times to values of 0, 47 g / L, while the expression level of Gp-41 protein increased to 10 %.

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Published

2020-03-04

How to Cite

Narciandi-Díaz, D. R. E., & Rivera-Martin, M. J. M. (2020). Production of Gp-41 (HIV-1) recombinant protein in E.coli: effect of magnetic field. Revista Cubana De Química, 32(1), 1–17. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5123

Issue

Vol. 32 No. 1 (2020)

Section

Artículos

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