Production of Gp-41 (HIV-1) recombinant protein in E.coli: effect of magnetic field
Keywords:E. coli, Gp-41, HIV-1, fed batch culture, magnetic field
The influence of the extremely low frequency electromagnetic field was studied in a fed batch culture of Escherichia coli (E.coli), developed for the production of the recombinant Gp-41 protein of human immunodeficiency virus type 1 (HIV-1). An electromagnet, which generates non-homogeneous magnetic fields, was used, at a constant induction value of 0.055 Tesla, and exposure time of 6, 5 h. With the application of the magnetic field, a 1, 2-fold increase in volumetric production (320 mg / L) and 1,5 times in process productivity (30 mg / L) were obtained. At the same time, acetate production decreased 20 times to values of 0, 47 g / L, while the expression level of Gp-41 protein increased to 10 %.
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