Evaluation of the anti-angiogenic activity of methyl jasmonate in chorioallantoic membrane of chicken embryos by means of digital image analysis

Authors

  • Miriela Milagros Escobedo-Nicot Facultad de Ciencias Naturales y Exactas, Universidad de Oriente, Cuba
  • Guillermo Mojícar-Martínez Facultad de Ciencias Naturales y Exactas, Universidad de Oriente, Cuba
  • Wilkie Ernesto Delgado-Font Facultad de Ciencias Naturales y Exactas, Universidad de Oriente, Cuba
  • Leandro Ticlia de la Cruz Instituto Oceanográfico, Universidade de São Paulo, Brasil
  • Ligia Ferreira-Gomes Laboratório de Microrreologia e Fisiologia Molecular, Faculdade de Ciências Farmacêuticas e Instituto de Física, Universidade de São Paulo, Brasil

Keywords:

quantification; angiogenesis; methyl jasmonate; CAM.

Abstract

A study is presented to measure the effect of Methyl Jasmonate (MJ) on angiogenesis in chori-oallantoic membrane (CAM) samples from chicken embryos, using an imaging analysis software. The characteristics considered are: terminal points, bifurcation points, area density, vascular length and fractal dimension, among others. In addition, the classification of blood vessels using Strahler and Tokunaga taxonomies is included, which allows classifying segments of hierarchical structures. For the evaluation of the results, control samples and others treated with MJ in water and liposomes are used in three concentrations 7.5, 15 and 30 μM. The anti-angiogenic tendency was corroborated in the environment of liposomes considering the decrease of the quantitative values of all the evaluated characteristics with regard to the control samples. The results obtained agree with data from literature regarding the best bioavailability associated to the lipid vehicle.

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Published

2021-01-06

How to Cite

Escobedo-Nicot, M. M., Mojícar-Martínez, G., Delgado-Font, W. E., Ticlia de la Cruz, L., & Ferreira-Gomes, L. (2021). Evaluation of the anti-angiogenic activity of methyl jasmonate in chorioallantoic membrane of chicken embryos by means of digital image analysis. Revista Cubana De Química, 32(3), 527–542. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5161

Issue

Vol. 32 No. 3 (2020)

Section

Artículos

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