Evaluation of the anti-angiogenic activity of methyl jasmonate in chorioallantoic membrane of chicken embryos by means of digital image analysis
Keywords:quantification; angiogenesis; methyl jasmonate; CAM.
A study is presented to measure the effect of Methyl Jasmonate (MJ) on angiogenesis in chori-oallantoic membrane (CAM) samples from chicken embryos, using an imaging analysis software. The characteristics considered are: terminal points, bifurcation points, area density, vascular length and fractal dimension, among others. In addition, the classification of blood vessels using Strahler and Tokunaga taxonomies is included, which allows classifying segments of hierarchical structures. For the evaluation of the results, control samples and others treated with MJ in water and liposomes are used in three concentrations 7.5, 15 and 30 μM. The anti-angiogenic tendency was corroborated in the environment of liposomes considering the decrease of the quantitative values of all the evaluated characteristics with regard to the control samples. The results obtained agree with data from literature regarding the best bioavailability associated to the lipid vehicle.
How to Cite
This journal provides immediate open access to its content, based on the principle that offering the public free access to research helps a greater global exchange of knowledge. Each author is responsible for the content of each of their articles.