UNIÓN DE LECTINAS AL BORDE EN CEPILLO DE VELLOSIDADES INTESTINALES: HERRAMIENTA PARA LA EVALUACIÓN DE LA GLICOSILACIÓN INTESTINAL

Authors

  • M. Sc. Gregory R. Valdés-Paneca Facultad de Ciencias Agropecuiarias, Universidad de Sancti Spiritus "José Martí Pérez"
  • Dr.C. Luis O. Maroto-Martín Facultad de Ciencias Agropecuarias, Universidad Central de las Villas
  • Dr.MV. Eduardo Cruz-Muñoz Facultad de Ciencias Agropecuarias, Universidad Central de las Villas
  • M. Sc. Pedro de la Fe-Rodríguez Facultad de Ciencias Agropecuarias, Universidad Central de las Villas
  • M. Sc. Yaima Hernández-Beltrán Facultad de Ciencias Agropecuiarias, Universidad de Sancti Spiritus "José Martí Pérez"

Abstract

This research was conducted as a first step in the analysis of intestinal glycosylation process and the relationship of this with the host-pathogen interaction, aiming to test the binding of plant lectins to brush border of the small intestine. The effect of different plant lectins block the adhesion of E. coli expressing
enterotoxigenic F4 fimbriae. Two experiments were conducted. First - the villi were incubated with lectin of Glycine max, Ulex europaeus, Lens culinaris and Arachis hypogaea at different concentrations and were then incubated with the strain of E. coli GIS26 (F4AC). Second - twenty-six biotinylated plant lectins
faced villi in 3 different fixation methods. The binding was verified by immunofluorescence using streptavidin-fluorescein as indicator. It is proved that: for the first case there was complete blockage of the adhesion of E. coli GIS26 the brush border by lectins, for the second case there was moderate to strong adhesion to intestinal epithelial cells in 69,2 % (18/26) of lectins. At the same time tested the biotinylated lectin adhesion to different regions of the small intestine and the union of the fimbrial antigens F4ab, F4AC and F4ad the brush border. Differences were found in the binding profile of lectins along different regions of the small intestine and non-homogeneity in the adhesion of the fimbrial antigens.
Keywords: intestinal glycosylation process, plant lectins.

Downloads

Published

2015-11-04

How to Cite

Valdés-Paneca, M. S. G. R., Maroto-Martín, D. L. O., Cruz-Muñoz, D. E., de la Fe-Rodríguez, M. S. P., & Hernández-Beltrán, M. S. Y. (2015). UNIÓN DE LECTINAS AL BORDE EN CEPILLO DE VELLOSIDADES INTESTINALES: HERRAMIENTA PARA LA EVALUACIÓN DE LA GLICOSILACIÓN INTESTINAL. Revista Cubana De Química, 24(1), 27–31. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/295

Issue

Vol. 24 No. 1 (2012)

Section

Artículos

License

This journal provides immediate open access to its content, based on the principle that offering the public free access to research helps a greater global exchange of knowledge. Each author is responsible for the content of each of their articles.