Propiedades fitoquímicas y antibacterianas de extractos de Tagetes erecta L. (Asteraceae)
Abstract
El presente trabajo tuvo como objetivo evaluar las propiedades fitoquímicas y antibacterianas de extractos de hojas y flores de Tagetes erecta L. (flor de muerto). Se colectan plantas presentes en el campus de la Universidad de Matanzas y las muestras se lavan, secan y trituran. Se realizan extractos acuosos y etanólicos y se determina las cantidades relativas de varios metabolitos secundarios. Se cuantifica el contenido de azúcares reductores, carbohidratos y proteínas solubles totales. Se evalúa la actividad antibacteriana contra cepas de referencia y salvajes. Se observó la presencia notable de flavonoides, terpenos, taninos, cumarinas y glucósidos cardiotónicos. Los extractos etanólicos de hojas y flores mostraron propiedades antibacterianas frente a Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae y Staphylococcus epidermidis, con valores inhibitorios superiores en las flores. Los resultados obtenidos justifican el uso folclórico de esta especie y las potencialidades para el control de enfermedades bacterianas.
Palabras clave: Tagetes, fitoquímica, actividad antibacteriana, metabolitos secundarios.
References
EKALUO, U. B.; IKPEME, E. V.; EKERETTE, E. E.; CHUKWU, C. I. “In vitro antioxidant and free radical activity of some Nigerian medicinal plants: bitter leaf (Vernonia amygdalina L.) and guava (Psidium guajava Del.)”. Research Journal of Medicinal Plant. 2015, 9 (5), 215-226. ISSN 1819-3455.
MASOKO, P. “Phytochemical analysis, antioxidant and antibacterial properties of Spilanthes mauritiana used traditionally in Limpopo Province, South Africa”. Journal of Evidence-Based Complementary & Alternative Medicine. 2017, 22 (4), 936-943. DOI: 11.1177/2515690x17746774.
EKALUO, U. B.; IKPEME, E. V.; UDENSI, O. U.; EKERETTE, E. E. et al. “Comparative in vitro assessment of drumstick (Moringa oleifera) and neem (Azadirachta indica) leaf extracts for antioxidant and free radical scavenging activities”. Res. J. Med. Plant. 2015, 9 (1), 24-33. ISSN 1819-3455.
MUNITA, J.M. AND ARIAS, C.A. “Mechanisms of Antibiotic Resistance”. Microbiol Spectr. 2016, 4 (2), 1-37. DOI:10.1128/microbiolspec.VMBF-0016-2015.
DJEUSSI, D. E.; NOUMEDEM, J. A.; SEUKEP, J. A.; FANKAM, A. G. “Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria”. BMC, Complement Altern Med. 2013, 13 (1), 1-9. DOI: 10.1186/1472-6882-13-164.
WIKANINGTYAS, P.; SUKANDAR, E. Y. “The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens”. Asian Pacific Journal of Tropical Biomedicine. 2016, 6 (1), 16–19. ISSN 2221-1691.
KADAM, P. V.; BHINGARE, C. L.; SUMBE, R. B.; NIKAM, R. Y.; PATIL, M. J. “Pharmacognostic, physicochemical and phytochemical investigation of Tagetes erecta Linn flowers (Asteraceae)”. Journal of Biological and Scientific Opinion. 2013, 1 (3), 21- 24. DOI: 10.7897/2321-6328.01124.
SHETTY, L. J.; SAKR, F. M.; AL OBAIDY, K.; PATEL, M. J.; SHAREEF, H. “A brief review on medicinal plant Tagetes erecta Linn”. Journal of Applied Pharmaceutical Science. 2015, 5 (3), 091-095. ISSN 2231-3354.
KWAMBOKA, N. J.; NGWELA, W. J.; MORWANI, G. R. “In vitro antibacterial activity of Tagetes minuta and Capsicum frutescens extracts against Pectobacterium carotovorum”. International Journal of Agricultural Sciences. 2016, 6 (8), 1119-1127. ISSN: 2167-0447.
RHAMA, S.; MADHAVAN, S. “Antibacterial Activity of the Flavonoid-patulitrin isolated from the flowers of Tagetes erecta L.”. International Journal of Pharm. Tech Research. 2011, 3 (3), 1407-1409. ISSN 0974-4304.
SARIN, R. “Insecticidal activity of callus culture of Tagetes erecta”. Fitoterapia. 2004, 75 (1), 62-64. DOI: 10.1016/j.fitote.2003.07.011.
NIRANJAN, K.; SATHIYASEELAN, V.; JEYASEELAN, E. C. “Screening for antimicrobial and phytochemical properties of different solvents extracts of leaves of Pongamia pinnata”. International Journal of Scientific and Research Publications. 2013, 3 (1), 1-3. ISSN 2250-3153.
PAREKH, J.; CHANDA, S. “Antibacterial and phytochemical studies on twelve species of Indian medicinal plants”. African Journal of Biomedical Research. 2006, 10, 175- 181. ISSN 1119-5096.
CHIGODI, M. O.; SAMOEI, D. K.; MUTHANGYA, M. “Phytochemical screening of Agave sisalana perrine leaves (waste)”. International Journal of Applied Biology and Pharmaceutical Technology. 2013, 4 (4), 200-204. ISSN 0976-4550.
MINISTERIO DE SALUD PÚBLICA (MINSAP). Guía metodológica para la investigación en plantas medicinales. La Habana: Editorial Pueblo y Educación, 1997.
MILLER, G. “Use of dinitrosalicylic acid reagent for determination of reducing sugar”. Anal. Chem. 1959, 31 (3), 426-428. DOI: 10.1021/ac60147a030.
DUBOIS, M. K.; GILLES, A.; HAMILTON, J.K.; REBERS, P.A.; SMITH, F. “Colorimetric method for determination of sugars and related substance”. Anal. Chem, 1956, 28 (3), 350-356. Disponible en: https://pdfs.semanticscholar.org/4c90/084da99ed7c2099fba34a2becc4b41d70ce1.pdf. Consulta: 7 de noviembre, 2018.
LOWRY, O. H.; ROSEBROUGH, N. J.; FARR, A. L.; RANDALL, R. “Protein measurement the Folin phenol reagent”. J. Biol. Chem. 1951, 193 (1), 265-275. Disponible en: https://www.semanticscholar.org/paper/Protein-Measurement-with-the-Folin-Lowry-ROSEBROUGH/0dea7761d3db2a1b723f94dfb03735848ace4799. Consulta: 7 de noviembre de 2018.
PEREZ, C.; PAUL, M.; BAZERQUE, P. “An antibiotic assay by the agar well diffusion method”. Acta Bio. Med. Exp. 1990, 15, 113- 115.
SIGARROA, A. Biometría y Diseño Experimental. La Habana: Editorial Pueblo y Educación, 1985.
DEVIKA, R.; KOILPILLAI, J. “Phytochemical screening studies of bioactive compounds of Tagetes erecta”. Int. J. Pharm. Bio. Sci. 2012, 3 (4), 596-602. ISSN 0975-6299.
LIU, W.; LIU, J.; YIN, D.; ZHAO, X. Influence of Ecological Factors on the Production of Active Substances in the Anti-Cancer Plant Sinopodophyllum hexandrum (Royle) T.S. Ying. PLos One. 2015, 10 (4), 1-22. DOI:10.1371/journal.pone.0122981.
REDDY, G. K.; LAKSHMI, S. M.; KUMAR, C. K. A.; KUMAR, D. S.; SRINIVAS, T.L. “Evaluation of anti-inflammatoy and antioxidant activity of methanolic extract of Agave cantala Roxb”. Journal of Global Trends in Pharmaceutical Sciences. 2013, 4 (4), 1300-1309. ISSN 2230-7346.
ABUBACKER, M. N.; SATHYA, C.; PRABAKARAN, R. “In vitro antifungal potentials of Hamelia patens Jacq. (Rubiaceae) aqueous extracts of leaves, flowers and fruits”. Biosciences Biotechnology Research Asia. 2013, 10 (2), 699-704.
AL MAJEDY, Y. K.; AL AMIERY, A.; KADHUM, A. A.; BAKARMOHAMAD, A. “Antioxidant activity of coumarins”. Systematic Reviews in Pharmacy. 2017, 8 (1), 24-30. DOI: 10.5530/srp.2017.1.6.
GUERRA, L. J. O.; NOGUEIRAS, C.; DELGADO, R.; HERNÁNDEZ, O. “Determinación cuantitativa de saponinas y azúcares reductores de Agave briottoniana T”. Revista Cubana de Química. 2001, 13 (3), 37-42.
MADIGAN, M. T.; MARTINKO, J. M.; BENDER, K. S.; BUCKLEY, B.; STAHL, D. A. Brock Biology of Microorganism. 14ta edición. New York: McGraw Hill Medical. 2015. ISBN: 9780321897398.
VERMA, P. AND VERMA, A. “Evaluation of antibacterial activity of different parts of Tagetes erecta”. International Journal of Pharmacy and Life Sciences. 2012, 3 (6), 1766-1768. ISSN: 0976-7126.
RAMYA, R.; MAHNA, S.; BHANUMATHI, S. P.; BHAT, S. K. “Analysis of phytochemical composition and bacteriostatic activity of Tagetes spp”. Inter. Res. J. Pharm. 2012, 3 (11), 114-116. ISSN 2230-8407.
KHATTAK, K.F. “Antioxidant activities and phytochemicals of Tagetes erecta flowers as affected by drying methods”. J. Appl. Environ. Biol. Sci. 2014, 4 (9), 253-262. ISSN 2090-4274.
JIMÉNEZ SUÁREZ, V.; REYES MUNGUÍA, A.; PÉREZ BERÚMEN, C.; ALVARADO SÁNCHEZ, B. “Separación cromatográfica del extracto de Hamelia patens”. Revista Académica de Investigación. 2012, 11, 1-10. ISSN: 1989-9300.
Downloads
Published
How to Cite
Issue
Section
License
This journal provides immediate open access to its content, based on the principle that offering the public free access to research helps a greater global exchange of knowledge. Each author is responsible for the content of each of their articles.
