Magnetic iron oxide nanoparticles for plasmid DNA isolation


  • Loreley Morejón-Alonso Departamento de Química General e Inorgánica, Facultad de Química, Universidad de La Habana, Cuba
  • Liem Wilson-Savón Instituto de Ciencias del Mar, La Habana, Cuba
  • Julio R. Fernández-Masso Dpto de Biología de Sistemas. Centro de Ingeniería Genética y Biotecnología, Cuba
  • Carlos Díaz-Águila Centro de Biomateriales, Universidad de La Habana, La Habana, Cuba


nanoparticles; magnetite; silica; Stöber; DNA extraction.


The aim of this work was the synthesis and characterization of iron oxide magnetic nanoparticles coated with silica (Fe3O4@SiO2) intended for plasmid DNA isolation. Synthesis was carried out through co-precipitation method and coating was performed used the Stöber method. Materials were characterized by X-ray fluorescence, X-ray diffraction and Infrared spectroscopy. DNA purification was performed by comparing the ethanolic precipitation method, the QIAprep® commercial DNA purification kit and the synthesized Fe3O4@SiO2. Results showed that is possible to obtain Fe3O4@SiO2 (ranging size from 9 to 12 nm) through the proposed methodology. The amount of Si adsorbed onto the surface of magnetic particles and the crystal size of the Fe3O4@SiO2 were pH and washing-conditions dependent. The isolation assay showed that it is possible to recovery efficiently plasmid DNA using the Fe3O4@SiO2 obtain in normal conditions as solid support.



How to Cite

Morejón-Alonso, L., Wilson-Savón, L., Fernández-Masso, J. R., & Díaz-Águila, C. (2020). Magnetic iron oxide nanoparticles for plasmid DNA isolation. Revista Cubana De Química, 32(2), 195–217. Retrieved from