Magnetic iron oxide nanoparticles for plasmid DNA isolation

Authors

  • Loreley Morejón-Alonso Departamento de Química General e Inorgánica, Facultad de Química, Universidad de La Habana, Cuba
  • Liem Wilson-Savón Instituto de Ciencias del Mar, La Habana, Cuba
  • Julio R. Fernández-Masso Dpto de Biología de Sistemas. Centro de Ingeniería Genética y Biotecnología, Cuba
  • Carlos Díaz-Águila Centro de Biomateriales, Universidad de La Habana, La Habana, Cuba

Keywords:

nanoparticles; magnetite; silica; Stöber; DNA extraction.

Abstract

The aim of this work was the synthesis and characterization of iron oxide magnetic nanoparticles coated with silica (Fe3O4@SiO2) intended for plasmid DNA isolation. Synthesis was carried out through co-precipitation method and coating was performed used the Stöber method. Materials were characterized by X-ray fluorescence, X-ray diffraction and Infrared spectroscopy. DNA purification was performed by comparing the ethanolic precipitation method, the QIAprep® commercial DNA purification kit and the synthesized Fe3O4@SiO2. Results showed that is possible to obtain Fe3O4@SiO2 (ranging size from 9 to 12 nm) through the proposed methodology. The amount of Si adsorbed onto the surface of magnetic particles and the crystal size of the Fe3O4@SiO2 were pH and washing-conditions dependent. The isolation assay showed that it is possible to recovery efficiently plasmid DNA using the Fe3O4@SiO2 obtain in normal conditions as solid support.

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Published

2020-09-29

How to Cite

Morejón-Alonso, L., Wilson-Savón, L., Fernández-Masso, J. R., & Díaz-Águila, C. (2020). Magnetic iron oxide nanoparticles for plasmid DNA isolation. Revista Cubana De Química, 32(2), 195–217. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5135

Issue

Vol. 32 No. 2 (2020)

Section

Artículos

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