Conserved insertions in protein sequences for molecular studies in the genus Rhodomicrobium
Keywords:Rhodomicrobium; identificación; marcadores moleculares; indeles.
The genus Rhodomicrobium comprises species with agricultural and environmental importance. However, few investigations have been conducted to identify molecular markers that could be used to distinguish members of Rhodomicrobium from other groups of bacteria. Conserved signature indels (insertions and deletions) in protein sequences are useful for evolutionary and taxonomic studies in bacteria. Homologous sequences of the proteins NAD+ dependent DNA ligase and Serine-tRNA synthetase were obtained from the UniprotKB/Swiss-Prot database and aligned using MUSCLE programme. Phylogenomic analysis was performed through the Maximum Likelihood method with RAxML. Insertions were identified which support the monophyly of this genus and confirm that Rhodomicrobium lacus forms a sister group of R. vannielii and R. udaipurense. Thus, the insertions analyzed constitute important molecular markers for taxonomic and evolutionary studies in Rhodomicrobium, and also for future biochemical or functional studies in those enzymes.
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