Inserciones conservadas en secuencias de proteínas para estudios moleculares en el género Rhodomicrobium

Autores

  • Ania Margarita Cutiño-Jiménez Centro de Estudios de Biotecnología Industrial (CEBI). Universidad de Oriente. Santiago de Cuba, Cuba
  • Andy Manuel González-Vicente Hospital Provincial Clínico Quirúrgico Docente “Saturnino Lora Torres”, Santiago de Cuba, Cuba

Palavras-chave:

Rhodomicrobium; identificación; marcadores moleculares; indeles.

Resumo

El género Rhodomicrobium comprende especies importantes para la agricultura y el medio ambiente. Sin embargo, pocas investigaciones se han dirigido a la identificación de marcadores moleculares que distingan a sus miembros de otros grupos de bacterias. Los marcadores Indeles (inserciones y deleciones) en secuencias de proteínas son útiles para estudios evolutivos y taxonómicos en bacterias. Se analizaron secuencias homólogas de las proteínas ADN ligasa NAD+ dependiente, y Serina ARNt sintetasa, obtenidas de la base de datos UniprotKB/Swiss-Prot, y posteriormente alineadas con el programa MUSCLE. El análisis filogenómico se realizó por el método de Máxima Verosimilitud con el programa RAxML. Se identificaron inserciones que soportan la monofilia del género y confirman que Rhodomicrobium lacus es un grupo hermano de R. vannielii y R. udaipurense. Las inserciones analizadas constituyen marcadores moleculares importantes para estudios taxonómicos y evolutivos en el género Rhodomicrobium, y para futuros estudios bioquímicos o funcionales en dichas enzimas.

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Publicado

2023-06-13

Como Citar

Cutiño-Jiménez, A. M., & González-Vicente, A. M. (2023). Inserciones conservadas en secuencias de proteínas para estudios moleculares en el género Rhodomicrobium. Revista Cubana De Química, 35(2), 185–199. Recuperado de https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5330

Edição

v. 35 n. 2 (2023)

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Artículos

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Copyright (c) 2023 Ania Margarita Cutiño-Jiménez, Andy Manuel González-Vicente

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