Production of fermentable sugars from rice straw
Keywords:
tratamiento hidrotermal; hidrólisis enzimática; paja de arroz; biomasa; etanol 2G.Abstract
Purpose: to present a method to enhance enzymatic hydrolysis of biomass, hydrothermal treatment. Methods: It was used Teflon bomb, fed with rice straw and water, to extract reducing sugars by hydrothermal treatment. Conditions were analysed using design of experiment procedure and ANOVA. The hydrothermal conditions were 140 and 200 °C, 20 and 60 min and 62,5 and 71,42 g straw per kilogram of water. The straw was filtered, dried and submitted to enzymatic hydrolysis; the conditions were 24 and 72 h, solid/liquid ratio were 0,625 and 2,50 % w/v and enzyme loading 16,5 and 66 FPU · g-1. Results: It was observed best yields of Reducing Sugars when submitting the Teflon bomb to 200 °C temperature for 60 min and 62,5 g straw per kg of water (pressure of 15,5 atm). The conditions for the enzymatic hydrolysis were 72 h and enzyme loading of 66 FPU · g-1. The enzymatic yield was 42,72 %.
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