Vancomycin Fluorescent Properties Studies to Develop a New HPLC Method Applied to Imprinted Polymer Characterization

Authors

  • Lic. Yanio E. Milián-Rodríguez Instituto de Ciencia y Tecnología de los Materiales (IMRE), La Habana
  • MSc. Alen N. Baeza-Fonte Instituto de Ciencia y Tecnología de los Materiales (IMRE), La Habana
  • Dr. Arístides C. Valdés-González Instituto de Ciencia y Tecnología de los Materiales (IMRE), La Habana

Abstract

The present work describes the analysis of the vancomycin fluorescence properties related to pH, kind and proportion of solvent and buffer nature over vancomycin fluorescence intensity in order to further development of a sensitive new method by high performance liquid chromatography with molecular fluorescence detection for its quantification. Practical conditions to measure vancomycin fluorescence were obtained in a solution of 1-butanol 5 %, pH 5 employing a KH2PO4/H3PO4(0,04 molL-1) buffer in
not continuous way. Measurements were acquired at 302 and 335nm of excitation and emission wavelengths, respectively. Knowledge obtained from these results was employed to develop a new high performance liquid chromatography method with fluorescence detection, using a Luna 5µ C18 (250 mmL · 2mm ID) column, with 0,15 mLmin-1 flow, adding 1-butanol post column. This method resulted linearbetween 0,01-10 µgmL-1 with detection and quantification limits of 2 and 5 ngmL-1, respectively. This method was successfully applied to determine vancomycin in water samples from the rebinding studies of molecular imprinted polymers experiments.
Keywords: vancomycin, antibacterial resistance, molecular fluorescence, HPLC.

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Published

2016-02-10

How to Cite

Milián-Rodríguez, L. Y. E., Baeza-Fonte, M. A. N., & Valdés-González, D. A. C. (2016). Vancomycin Fluorescent Properties Studies to Develop a New HPLC Method Applied to Imprinted Polymer Characterization. Revista Cubana De Química, 25(1), 32–41. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/807

Issue

Vol. 25 No. 1 (2013)

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Artículos

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