Serum and plasma samples deproteinization for analytical studies of carbamazepine


  • Yamilé Heredia-Díaz Universidad de Oriente
  • Roberto Machado-García Universidad de Oriente
  • Marllelyn Mendoza-Suárez Dirección Municipal de Salud Manuel Tames, Jamaica, Guantánamo
  • Deylis Jardines-Cala Centro de Toxicología y Biomedicina, Santiago de Cuba
  • Albis Vázquez-Domínguez Hospital Docente Clínico Quirúrgico Ginecobstétrico “Juan Bruno Zayas”


The quantitative determination of carbamazepine in human serum and plasma, it requires of a previous treatment to eliminate the proteins. In this study were used methanol and trichloroacetic acid to 10 %, with the purpose of selecting the protein precipitant that guarantees the biggest effectiveness to remove the proteins. The proportions serum/plasma-protein precipitant 1:2 for the methanol and 1:0,2 for the trichloroacetic acid were used. The evaluation of the efficiency of protein precipitation was carried out applying UV spectrophotometry; the spectra of the precipitated samples were registering in the range of 200-340 nm. The selection approach was the decrease from the absorbance intensity to the wave lengths of maximum absorption of the proteins. The methanol guaranteed a reduction of the absorbance in the region near at 235 nm and 280 nm as much in the serum as in the plasma. The trichloroacetic acid 10% didn't show effectiveness to remove the proteins.

Keyword: Carbamazepine, quantitative determination, serum, plasma, UV- spectrophotometry.

Author Biography

Yamilé Heredia-Díaz, Universidad de Oriente




How to Cite

Heredia-Díaz, Y., Machado-García, R., Mendoza-Suárez, M., Jardines-Cala, D., & Vázquez-Domínguez, A. (2016). Serum and plasma samples deproteinization for analytical studies of carbamazepine. Revista Cubana De Química, 28(3), 870–889. Retrieved from




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