Influence of Extraction Composition of volatile substances from the leaves of Zanthoxylum pistacifolium Griseb

Abstract

Zanthoxylum pistacifolium Griseb is an endemic plant that grows in the forests and hills of the south-Eastern cost of Cuba. In the reviewed bibliography none report regarding to the chemical composition, ethnobotanic and/or pharmacological information was find out. That is why the intention of this experimental work was to extract and to identify the volatile components of leaves collected in the locality of “el Palenque”, Siboney beach, Santiago de Cuba. The hydrodistillation-cohobation extraction method in three different mediums: neutral, acid and basic was used, while Gas Chromatography coupled to a Mass Spectrometry was considered for the chemical separation and characterization of the volatile compounds extracted.The acid and basic mediums increases the extraction yield but attempts against the structural integrity of the volatile compounds, that is why the conventional method was selected as the most effective. In neutral medium, 82 compounds (97,76 % of the total extracted) were identified, most of them belonging to terpene type. The main compounds isolated were α-Pinene (12,35 %), Linalool (6,68 %), (4E,6Z)-2,6-dimethyl-2,4,6-Octatriene (6,50 %), Limonene (6,19 %) and Phytol (6,06 %).

Keywords: Zanthoxylum pistacifolium Griseb, extraction, volatile compounds.