Obtaining a anti-CD45 leukocyte marker for flow cytometry

Authors

  • Grisel Rabell Velázquez Laboratorio de Anticuerpos y Biomodelos Experimentales (LABEX). Santiago de Cuba. Cuba.
  • Suyén Rodriguez Pérez Laboratorio de Anticuerpos y Biomodelos Experimentales (LABEX). Santiago de Cuba. Cuba.
  • Jeorge Fernández Duarte Laboratorio de Anticuerpos y Biomodelos Experimentales (LABEX). Santiago de Cuba. Cuba.
  • Yanet Rivero Fernández Laboratorio de Anticuerpos y Biomodelos Experimentales (LABEX). Santiago de Cuba. Cuba.
  • Katya Ramirez Tur Laboratorio de Anticuerpos y Biomodelos Experimentales (LABEX). Santiago de Cuba. Cuba.
  • Mercedes Cedeño Arias Centro de Inmunología Molecular. Habana. Cuba
  • Rancés Blanco Santana Centro de Inmunología Molecular. Habana. Cuba
  • Elio Cisneros Prego Laboratorio de Anticuerpos y Biomodelos Experimentales (LABEX). Santiago de Cuba. Cuba
  • Rosa María Pérez Silva Universidad de Oriente. Santiago de Cuba. Cuba

Abstract

The reactive anti-CD45 is useful for the diagnostic and the study of T or B cells immunodeficiency. In this works were assayed three protocols with the purpose to obtain this reagent for the production, each one modifying the relation among the fluorochrome and the antibody (F:P) to conjugate, referred to the proposal of 1 : 2 recommended. The antibody was purified by affinity chromatography with 99,3 % of purity; was evaluated the relation FITC/Protein to 495 nm and 280 nm; and also, was obtained the leukocyte recognition percent through cytometry of flow by three tried protocols which evidence good biological activity (90,7 % - 96,8 %). The best protocol was P-2, with the lowest production cost and highest profits compared with product imported usually used in health system. This study permitted establishes the process to obtain anti-CD45 - FITC conjugated in LABEX laboratories.

Keywords: monoclonal antibodies, leukocyte marker, Fluorescein isotiocyanate (FITC), flow cytometry.

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Published

2017-06-09

How to Cite

Rabell Velázquez, G., Rodriguez Pérez, S., Fernández Duarte, J., Rivero Fernández, Y., Ramirez Tur, K., Cedeño Arias, M., Blanco Santana, R., Cisneros Prego, E., & Pérez Silva, R. M. (2017). Obtaining a anti-CD45 leukocyte marker for flow cytometry. Revista Cubana De Química, 29(2), 284–302. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/2512

Issue

Vol. 29 No. 2 (2017)

Section

Artículos

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