Simultaneous production of ramnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa ORA9
Keywords:
rhamnolipids; polyhydroxyalkanoates; pseudomonas; kinetic.Abstract
In this work, the kinetics of the simultaneous production of rhamnolipids and polyhydroxyalkanoates from Pseudomonas aeruginosa ORA9 was studied. The kinetic parameters specific cell growth rate (μx), volumetric rate (rp) and specific rate of product formation (qP), and the yield of the product with respect to the biomass formed (YP/X) were determined. The results showed that polyhydroxyalkanoates production had a maximum accumulation of 22 % (1,14 g L-1) in the biomass, during the exponential phase of microbial growth. On the contrary, the highest yield of rhamnolipids is achieved at the end of the culture (YP/X = 1,024) with an increase in the kinetic variables evaluated after 18 hours, coinciding with the beginning of the stationary phase of microbial growth. It was found that, under the conditions tested, the culture time is an important factor that must be optimized to obtain adequate concentrations of both products.at the end of culture.
References
HORI, K.; MARSUDI, S.; UNNO, H. “Simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa”. Biotechnol.. Bioengin. 2002, 78, 699-707. ISSN: 1097-0290.
CIRSTEA, D. y col. “Use of some carbon sources by Pseudomonas strains for synthesizing polyhydroxyalkanoates and/or rhamnolipids”. Romanian Biotechnological Letters, 2014, 19, 3, 9400-9408. ISSN: 1224–5984.
RASHID, N. y col. “Dual-production of polyhydroxyalkanoate and rhamnolipid by Pseudomonas aeruginosa UMTKB-5 using industrial by-products”. Malaysian J. Anal. Sci., 2021, 25, 24-39. ISBN ISSN: 1394-2506.
NITSCHKE, M.; COSTA, S.; CONTIERO, J. “Rhamnolipids and PHAs: Recent reports on Pseudomonas-derived molecules of increasing industrial interest”. Process Biochemistry, 2011, 46, 3, 621-630. ISSN: 1359-5113.
ABDELMONEIM, H. y col. “Rhamnolipids: Preparation, Determination and Applications A Review”. American Journal of Food Science and Nutrition Research. 2017, 4, 1, 9-17, ISSN: 2381-621X (Print); ISSN: 2381-6228 (Online).
TORTAJADA, M.; FERREIRA DA SILVA, L.; PRIETO, M. A. “Second-generation functionalized medium-chain-length polyhydroxyalkanoates: the gateway to high-value bioplastic applications”. International Microbiology. 2013, 16, 1, 1-15. ISSN: 1139-6709.
ANJUM, A. y col. “Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements”. International Journal of Biological Macromolecules 2016, 89, 161–174. ISSN: 0141-8130, 1879-0003.
MOZEJKO-CIESIELSKA y col. “Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spot mutant: bioprocess characterization and transcriptome analysis”. AMB Express, 2017, 7, 92. doi: 10.1186/s13568-017-0396-z.
MANAVITEHRANI, I. y col. “Biomedical Applications of Biodegradable Polyesters”. Polymers 2016, 8, 20. ISSN: 0032-3861.
PANTAZAKI, A.; PAPANEOPHYTOU, CH.; LAMBROPOULOU, D. Simultaneous polyhydroxyalkanoates and rhamnolipids production by Thermus thermophiles HB8. AMB Express, 2011, 1, 17. ISSN: 2191-0855.
NOOR FAZIELAWANIE y col. “Dual-production of polyhydroxyalkanoate and rhamnolipid by Pseudomonas aeruginosa UMTKB-5 USING INDUSTRIAL BY-PRODUCTS”. Malaysian Journal of Analytical Sciences, 2021, 25, 1, 24 – 39. ISSN: 1394-2506.
IDOMEH, J. E. Co. “Production of Polyhydroxyalkanoates and Biosurfactants using Pseudomonas luteola”. Nigerian Journal of Basic and Applied Science, 2018, 26, 2, 67-75. ISSN: 0794-5698.
SAHARAN, B.; GREWAL, A.; KUMAR, P. “Biotechnological Production of Polyhydroxyalkanoates: A Review on Trends and Latest Developments”. Chinese Journal of Biology, 2014, 3, 1-18. doi: 10.1155/2014/802984.
RODRÍGUEZ, O. y col. “Screening and Characterization of Biosurfactant-Producing Bacteria Isolated from Contaminated Soils with Oily Wastes”. J. Environ. Treat. Tech., 2017, 5 (1), 5-11. ISSN: 2309-1185.
RAMSAY, B. y col. “Production of poly-β-hydroxybutyric-β-hydroxyvaleric acids”. Appl. Environ. Microbiol. 1990, 56, 2093–2098. ISSN: 0099-2240 (print); 1098-5336 (web).
VALENTIN, H.; REISER, S.; GRUYS, K. “Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) formation from γ-aminobutyrate and glutamate”. Biotechnology and Biongineering. 2000, 67, 3, 291-299. ISSN: 0006-3592 (print); 1097-0290 (web).
AIBA, S.; HUMPHREY, A. E.; MILLIS, N. F. Biochemical engineering, 2nd edn. New York: Academic Press, 1973. ISBN-10: 0120450526, ISBN-13: 978-0120450527.
RIIS, V.; MAI, W. “Gas chromatography determination of poly-bhydroxybutyric acid in microbial biomass-esther hydrochloric acid propanolisis”. J. Chromatogr. 1988. 445, 285-289. ISSN: 1873-3778.
LOPES, A. K. Produção de ramnolipídios por isolados de Pseudomonas: avaliação do efeito das fontes de carbono e nitrogênio na composição do ramnolípidio. 101p, Dissertação (Mestrado em Microbiologia) - Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo. 2011. Disponible en: https://www.teses.usp.br/teses/disponiveis/42/42132/tde-25052012 095221/publico/KarenLopesAlmeida_Mestrado_corrigida.pdf
STATGRAPHICS CENTURION, X.V.I. Statpoint Technologies. INC. version, 15.2, 17. 2009.
MARSUDI, S.; UNNO, H.; HORI, K. “Palm oil utilization for the simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa”. Appl Microbiol Biotechnol. 2008, 78, 955–961. ISSN (Online): 1432-0614.
COSTA, S. y col. “Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa”. Journal of Industrial Microbiology & Biotechnology, 2009, 36, 8, 1063–1072. Online ISSN 1476-5535 Print ISSN 1367-5435.
CHEN, F.; CHEN, CH.; RIADI, L.; JU, L. “Modeling RHL Quorum-Sensing Regulation on Rhamnolipid Production by Pseudomonas aeruginosa”. Biotechnol. Prog. 2004, 20, 1325-1331. ISSN (Online): 1520-6033.
DE LIMA, C. J. y col. “Biosurfactant Production by Pseudomonas aeruginosa Grown in Residual Soybean Oil.” Appl. Biochem. Biotechnol, 2009, 152, 156–168, ISSN: 2732289.
PERFUMO, A. y col. “Rhamnolipids are conserved biosurfactants molecules: implications
for their biotechnological potential”. Appl. Microbiol. Biotechnol., 2013, 97, 16, 7297-7306. ISSN (Online): 1432-0614.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Odalys Rodríguez-Gámez, Arelis Ábalos-Rodríguez, José Gregorio Cabrera-Gómez
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
This journal provides immediate open access to its content, based on the principle that offering the public free access to research helps a greater global exchange of knowledge. Each author is responsible for the content of each of their articles.