Production of fermentable sugars from rice straw
Keywords:tratamiento hidrotermal; hidrólisis enzimática; paja de arroz; biomasa; etanol 2G.
Purpose: to present a method to enhance enzymatic hydrolysis of biomass, hydrothermal treatment. Methods: It was used Teflon bomb, fed with rice straw and water, to extract reducing sugars by hydrothermal treatment. Conditions were analysed using design of experiment procedure and ANOVA. The hydrothermal conditions were 140 and 200 °C, 20 and 60 min and 62,5 and 71,42 g straw per kilogram of water. The straw was filtered, dried and submitted to enzymatic hydrolysis; the conditions were 24 and 72 h, solid/liquid ratio were 0,625 and 2,50 % w/v and enzyme loading 16,5 and 66 FPU · g-1. Results: It was observed best yields of Reducing Sugars when submitting the Teflon bomb to 200 °C temperature for 60 min and 62,5 g straw per kg of water (pressure of 15,5 atm). The conditions for the enzymatic hydrolysis were 72 h and enzyme loading of 66 FPU · g-1. The enzymatic yield was 42,72 %.
ADAMS, J. M.; GALLAGHER, J. A.; DONNISON, I. S. “Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments”. Journal of applied Phycology, v. 21, n. 5, p. 569, 2009. https://doi.org/10.1007/s10811-008-9384-7
ANP-AGÊNCIA NACIONAL DO PETRÓLEO, GÁS NATURAL E BIOCOMBUSTÍVEIS. Disponível em: http://www.anp.gov.br/wwwanp /biocombustiveis. Accessed 26 july 2020.
DE ANDRADE, E. T.; DE CARVALHO G., S. R.; DE SOUZA F., L. Programa do Proálcool e o etanol no Brasil. 2009. https://doi.org/10.22409/engevista.v11i2.236
SANTOS, F. et al. “Productive potential and quality of rice husk and straw for biorefineries”. Biomass Conversion and Biorefinery, v. 7, n. 1, p. 117-126, 2017. https://doi.org/10.1007/s13399-016-0214-x
GARROTE, G.; DOMÍNGUEZ, H.; PARAJO, J. C. “Hydrothermal processing of lignocellulosic materials”. European Journal of Wood and Wood Products, v. 57, n. 3, p. 191-202, 1999. https://www.researchgate.net/profile/GilGarrote/publication /226555890_Hydrothermal_processing_of_lignocellulosic_materials/links/0deec53126467304ad000000/Hydrothermal-processing-of-lignocellulosic-materials.pdf
YU, G. et al. “Pretreatment of rice straw by a hot-compressed water process for enzymatic hydrolysis”. Applied Biochemistry and Biotechnology, v. 160, n. 2, p. 539-551, 2010. https://doi.org/10.1007/s12010-008-8420-z
MOSIER, N. et al. “Features of promising technologies for pretreatment of lignocellulosic biomass”. Bioresource technology, v. 96, n. 6, p. 673-686, 2005a. https://doi.org/10.1016/j.biortech.2004.06.025
_____. “Optimization of pH controlled liquid hot water pretreatment of corn stover”. Bioresource technology, v. 96, n. 18, p. 1986-1993, 2005b. https://doi.org/10.1016/j.biortech.2005.01.013
WYMAN, C. E. et al. “Coordinated development of leading biomass pretreatment technologies”. Bioresource technology, v. 96, n. 18, p. 1959-1966, 2005. https://doi.org/10.1016/j.biortech.2005.01.010
OVEREND, R. P.; CHORNET, E. “Fractionation of lignocellulosics by steam-aqueous pretreatments”. Phil. Trans. R. Soc. Lond. A, v. 321, n. 1561, p. 523-536, 1987. https://doi.org/10.1098/rsta.1987.0029
LORA, E. E. S.; VENTURINI, O. J. Biocombustíveis, Volumes 1 e 2. Editora Interciência, Rio de Janeiro, 2012. ISBN: 9788571932289.
VAN SOEST, P. J.; WINE, R. H. “Development of a lignin and celulose in acid detergent fiber with permanganate”. Journal of the Association of Official Analytical Chemists, Baltimore, v. 51, p. 780-785, 1968. https://doi.org/10.1093/jaoac/51.4.780
VAN SOEST, P. J. “Use of detergents is the analysis of fibrous feeds II. A rapid method for the determination of fiber and lignin”. Journal of the Association of Official Analytical Chemists, Baltimore. v. 46, n. 5, p. 829-35, 1963. https://doi.org/10.1093/jaoac/46.5.829
_____. “Development of a compreheensive system of feed analysis and its application to forages”. Journal of Animal Science, Champaign, v. 26, n. 1, p. 119-128, 1967. https://doi.org/10.2527/jas1967.261119x
ADNEY, B.; BAKER, J. “Measurement of cellulase activities”. Laboratory analytical procedure, v. 6, p. 1996, 1996. https://www.researchgate.net/profile/ Shuddhodana-2/post/What-is-the-method-for-enzyme-pre-treatment-ofellulose/ attachment/5afa3fa8b53d2f63c3cbd242/AS%3A626370346504192 %40152634973 6627/download/Adney%2C+Nrel+-+2008+Measurement+of+Cellulase+Activities +Laboratory+Analytical+Procedure+%28+LAP+%29+Issue+Date+08+12+1996+Measurement+of.pdf
MILLER, G. L. “Use of dinitrosalicylic acid reagent for determination of reducing sugar”. Analytical chemistry, v. 31, n. 3, p. 426-428, 1959. https://doi.org/10.1021/ac60147a030
GREEN, R. H. Perry Donal W.; PERRY, R. H.; GREEN, D. W. Perry's chemical engineers' handbook. McGraw-Hill Professional Pub, New York, 2008. ISBN-13: 978-0071422949
WANG, X. et al. “Inhibitory effects of phenolic compounds of rice straw formed by saccharification during ethanol fermentation by Pichia stipites”. Bioresource technology, v. 244, p. 1059-1067, 2017. https://doi.org/10.1016/j.biortech.2017.08.096
SANTOS, F. A. et al. “Otimização do pré-tratamento hidrotérmico da palha de cana-de-açúcar visando à produção de etanol celulósico”. Química nova, v. 37, n. 1, p. 56-62, 2014. https://doi.org/10.1590/S0100-40422014000100011
DÍAZ, M. J. et al. « Hydrothermal pre-treatment of rapeseed straw”. Bioresource Technology, v. 101, n. 7, p. 2428-2435, 2010. https://doi.org/10.1016/j.biortech.2009.10.085
JIN, Sh.; CHEN, H. “Near-infrared analysis of the chemical composition of rice straw”. Industrial Crops and Products, v. 26, n. 2, p. 207-211, 2007. http://dx.doi.org/10.1016/j.indcrop.2007.03.004
VAZ JÚNIOR, S. et al. Análise química de biomassa. Embrapa, Brazil, 2015 Pag. 39, 1st edition. . ISBN-13: 978-8570354549
XIAO, X. et al. “Enhanced enzymatic hydrolysis of bamboo (Dendrocalamus giganteus Munro) culm by hydrothermal pretreatment”. Bioresource technology, v. 159, p. 41-47, 2014. https://doi.org/10.1016/j.biortech.2014.02.096
WEIL, J. R. et al. “Removal of fermentation inhibitors formed during pretreatment of biomass by polymeric adsorbents”. Industrial & engineering chemistry research, v. 41, n. 24, p. 6132-6138, 2002. https://doi.org/10.1021/ie0201056
SÁNCHEZ, B.; BAUTISTA, J. “Effects of furfural and 5-hydroxymethylfurfural on the fermentation of Saccharomyces cerevisiae and biomass production from Candida guilliermondii”. Enzyme and Microbial technology, v. 10, n. 5, p. 315-318, 1988. https://doi.org/10.1016/0141-0229(88)90135-4
SANTOS, JRA de; GOUVEIA, E. R. “Produção de bioetanol de bagaço de cana-de-açúcar”. Revista Brasileira de Produtos Agroindustriais, Campina Grande, v. 11, n. 1, p. 27-33, 2009. ISSN: 1517-8595.
KIM, Y.; MOSIER, N. S.; LADISCH, M. R. “Enzymatic digestion of liquid hot water pretreated hybrid poplar”. Biotechnology Progress, v. 25, n. 2, p. 340-348, 2009. https://doi.org/10.1002/btpr.137
How to Cite
Copyright (c) 2023 Miral Miranda-Neto, Rodrigo de Faria-Rodrigues, Walter Augusto-Ruiz, Paulo Henrique-Beck, Toni Jefferson-Lopes
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
This journal provides immediate open access to its content, based on the principle that offering the public free access to research helps a greater global exchange of knowledge. Each author is responsible for the content of each of their articles.