Development of pH indicator films based on chitosan and beeswax


  • Roberto Sáez-Gastón Centro de Biomateriales (BIOMAT). Universidad de La Habana. La Habana, Cuba
  • Dairon Iglesias-Guevara Instituto de Farmacia y Alimentos (IFAL). Universidad de La Habana, La Habana, Cuba
  • Alicia Casariego-Año Instituto de Farmacia y Alimentos (IFAL). Universidad de La Habana, La Habana, Cuba
  • Carlos A. Peniche-Covas Facultad de Química (FQ). Universidad de La Habana. La Habana, Cuba
  • Yaimara Solís-Moré Centro de Biomateriales (BIOMAT). Universidad de La Habana. La Habana, Cuba


quitosano; rojo fenol; cera de abejas; tinte polimérico; películas indicadoras de pH.


A pH-sensitive polymeric dye was synthesized by grafting phenol red onto chitosan using a simple methodology. Successful grafting is confirmed by physical-chemical techniques. From the polymeric dye obtained, pH indicator films were developed with the addition of beeswax. The influence of the concentration of chitosan and beeswax on the humidity and water vapor permeability of the films was evaluated. The lowest moisture values of the formulated films were obtained for lower concentrations of wax and chitosan. However, the permeability was lower at higher wax content, regardless of the chitosan content. The colorimetric capacity of the best film obtained changed in the expected pH range and in contact with shrimp used as model food. Therefore, the films obtained suggest their possible food applications.


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How to Cite

Sáez-Gastón, R., Iglesias-Guevara, D., Casariego-Año, A., Peniche-Covas, C. A., & Solís-Moré, Y. (2024). Development of pH indicator films based on chitosan and beeswax. Revista Cubana De Química, 36(1), 1–14. Retrieved from