Antifungal compounds identification in Plectranthus amboinicus (Lour) Spreng leaf extracts

Authors

  • Yoannia G. Pupo-Blanco Centro de Estudios de Química Aplicada, Facultad de Ciencias Técnicas. Universidad de Granma, Cuba
  • Eleuterio Burgueño-Tapia Departamento Química Orgánica. Escuela Nacional de Ciencias Biológicas. Instituto Politécnico Nacional, México
  • Dina I. Malheiros-Dinis de Mendonca Fiber Material and Environmental Technologies, Universidade da Beira Interior, Portugal
  • Belyani Vargas-Batis Departamento de Agronomía, Campus Julio Antonio Mella, Universidad de Oriente, Santiago de Cuba, Cuba

Keywords:

Plectranthus amboinicus; Alternaria porri; flavonoides; terpenoides; antifúngico.

Abstract

This work aimed to identify compounds with antifungal activity against Alternaria porri present in leaf
extracts of French oregano (Plectranthus amboinicus). The crude extract was partitioned consecutively into
hexane, chloroform, ethyl acetate, and sterile distilled water. The bioactivity of the fractions was evaluated
using agar dilution and hanging drop techniques for mycelium and spores, respectively. Compounds were
separated from the most active fractions using column chromatography and thin layer chromatography.
The isolated compounds were analyzed by 1H and 13C Nuclear Magnetic Resonance. The chloroformic
and hexanic fractions were the most active, managing to reduce mycelial growth by about 50 % and inhibit
conidial germination by more than 90 %. From these fractions, seven compounds (flavonoids and terpenoids)
were identified, of which six showed antifungal activity, as did the oil obtained from the hexanic fraction.

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Published

2024-12-11

How to Cite

Pupo-Blanco, Y. G., Burgueño-Tapia, E., Malheiros-Dinis de Mendonca, D. I., & Vargas-Batis, B. (2024). Antifungal compounds identification in Plectranthus amboinicus (Lour) Spreng leaf extracts. Revista Cubana De Química, 36(1), 10–21. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5361

Issue

Vol. 36 No. 1 (2024)

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Artículos

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Copyright (c) 2024 Yoannia G. Pupo-Blanco, Eleuterio Burgueño-Tapia, Dina I. Malheiros-Dinis de Mendonca, Belyani Vargas-Batis

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