A Two-Step Purification Procedure of Phospholipases A2 from the Sea Anemone Condylactis Gigantea

Authors

  • Frenkel Guisado−Bourzac Universidad de Oriente
  • Dolores Lázara Romero−Del−Sol Centro de Estudio de Proteínas (CEP), Facultad de Biología, Universidad de La Habana
  • José Manuel Guisán−Seijas Instituto de Catálisis y Petroleoquímica, CSIC, Campus UAM, Madrid, España
  • Jorge González−Bacerio Centro de Estudio de Proteínas (CEP), Facultad de Biología, Universidad de La Habana
  • Joaquín Díaz−Brito Centro de Estudio de Proteínas (CEP), Facultad de Biología, Universidad de La Habana
  • Andreimar Martins−Soares Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, Porto Velho-RO, Brazil
  • Alberto del Monte−Martínez Centro de Estudio de Proteínas (CEP), Facultad de Biología, Universidad de La Habana

Abstract

Marine coelenterate venom consists of complex mixtures of several substances, mainly proteins, for which phospholipase A2 activity has been described. This research study aims to test a two-step purification procedure for phospholipases A2 (PLA2) to filter enzymes for further characterization. PLA2 purification from the sea anemone Condylactis gigantean is conducted through a chromatographic affinity support MANA - Sepharose CL 4B with covalently immobilized phosphatidylcholine egg. The phospholipase A2 activity was verified by using qualitative TLC and a fluorogenic substrate. By means of the above-mentioned support, purification of three protein-based components can be conducted. These components obtained have molecular weights between 18000 and 14000, and at least one component possessing phospholipase A2 activity.

Keywords: affinity, phospholipase A2, Condylactis gigantea, sea anemone, protein purification.

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Published

2017-01-16

How to Cite

Guisado−Bourzac, F., Romero−Del−Sol, D. L., Guisán−Seijas, J. M., González−Bacerio, J., Díaz−Brito, J., Martins−Soares, A., & Monte−Martínez, A. del. (2017). A Two-Step Purification Procedure of Phospholipases A2 from the Sea Anemone Condylactis Gigantea. Revista Cubana De Química, 29(1), 133–149. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/1997

Issue

Vol. 29 No. 1 (2017)

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Artículos

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