INMOVILIZACIÓN DE CELULASA SOBRE UNA MATRIZ DE QUITINA-QUITOSANA

Authors

  • Sheila Romo Departamento de Química Analítica y Tecnología de los Alimentos, Universidad de Castilla, Ciudad Real, España
  • Conrado Camacho Centro de Biotecnología, Universidad de Matanzas
  • Reynaldo Villalonga-Santana Departamento de Química Analítica, Facultad de Química, Universidad Complutense de Madrid, España
  • Juan Úbeda-Iranzo Departamento de Química Analítica y Tecnología de los Alimentos, Universidad de Castilla, Ciudad Real, España
  • María Arevalo-Villena Departamento de Química Analítica y Tecnología de los Alimentos, Universidad de Castilla, Ciudad Real, España
  • Ana Isabel Briones-Pérez Departamento de Química Analítica y Tecnología de los Alimentos, Universidad de Castilla, Ciudad Real, España
  • Héctor L. Ramírez Centro de Biotecnología, Universidad de Matanzas

Abstract

Cellulase from Biochemika were inmovililized for physics absorption and covalent bond with glutaraldehyde in the support chitin-chitosan. The optimal concentration of glutaraldehyde for bond enzyme to the support was 0,25 % with reaction time ½ hour. The best conditions for the physics absorption were: pH= 4,5, protein concentration = 170 μ/mL and incubation time= 4 hours. Various
characteristics of immobilized cellulases such as the pH optimum , thermal stability, and reuse were evaluated. The modification process increased the thermostability of enzymes after physics adsorption on support and covalent attachement respectively. The pH optimum of enzymes change for the acid region for modification. The cellulase immobilized by covalent attachment to the chitosan-coated chitin support, retained about 60 % of its initial activity after 15 cycles of reuse.

Keywords: cellulase, immobilized, covalent bond, physics absorption, chitin-chitosan.

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Published

2015-11-04

How to Cite

Romo, S., Camacho, C., Villalonga-Santana, R., Úbeda-Iranzo, J., Arevalo-Villena, M., Briones-Pérez, A. I., & Ramírez, H. L. (2015). INMOVILIZACIÓN DE CELULASA SOBRE UNA MATRIZ DE QUITINA-QUITOSANA. Revista Cubana De Química, 24(1), 57–64. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/312

Issue

Vol. 24 No. 1 (2012)

Section

Artículos

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