Optimization of lipid production from whey and molasses by an oleaginous yeast strain
Keywords:
biodiesel; lipids; molasses; whey; agroindustrial by-products.Abstract
The aim of this work was the optimization of lipid production by an oleaginous yeast strain using agroindustrial by-products obtained from the dairy and sugar industries as carbon sources. Screening (Plackett-Burman) and optimization (Box-Behnken) designs were used to determine the influence of the components of the culture medium on the percentage of accumulated lipids and the biomass concentration, as well as to optimize the composition of the culture medium, respectively. The triglycerides concentration was determined by the sulfo-phospho-vanillin method. The regression equations obtained during optimization predicted maximum values of lipid accumulation of 65 % and biomass concentration of 13 mg/mL, results comparable to those obtained by other researchers and which point to the industrial applicability of this strain in technological systems aimed at biodiesel production.
References
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24. JIRU, T. M. et al. “Production of single cell oil from cane molasses by Rhodotorula kratochvilovae (syn. Rhodosporidium kratochvilovae) SY89 as a biodiesel feedstock”, Chemistry Central Journal. 2018, 12, 91.DOI: 10.1186/s13065-018-0457-7.
2. SCHNEIDER, T.et al. “Utilization of soluble starch by oleaginous red yeast Rhodotorula glutinis”, Journal of Sustainable Bioenergy Systems. 2013, 3, 57-63. DOI:10.4236/jsbs.2013.31007.
3. TAKAKU, H. et al. “Lipid metabolism of the oleaginous yeast Lipomyces starkeyi”, Applied Microbiology and Biotechnology. 2020, 104, 6141–6148. DOI: 10.1007/s00253-020-10695-9.
4. KAMINENI, A. and SHAW, J. “Engineering triacylglycerol production from sugars in oleaginous yeasts”, Current Opinion in Biotechnology. 2020, 62, 239–247. DOI: 10.1016/j.copbio.2019.12.022
5. AGEITOS, J. M. et al. “Oily yeasts as oleaginous cell factories”, Applied Microbiology and Biotechnology. 2011, 90 (4), 1219-1227. DOI: 10.1007/s00253-011-3200-z.
6. SAENGE, C. et al. “Efficient concomitant production of lipids and carotenoids by oleaginous red yeast Rhodotorula glutinis cultured in palm oil mill effluent and application of lipids for biodiesel production”, Biotechnology and Bioprocess Engineering. 2011, 16, 23-33. DOI: 10.1007/s12257-010-0083-2.
7. KITCHA, S.; CHEIRSILP, B. “Screening of oleaginous yeasts and optimization for lipid production using crude glycerol as a carbon source”, Energy Procedia. 2011, 9, 274-282, DOI: 10.1016/j.egypro.2011.09.029.
8. MANOWATTANA, A. et al. “Bioconversion of biodiesel-derived crude glycerol into lipids and carotenoids by an oleaginous red yeast Sporidiobolus pararoseus KM281507 in an airlift biorreactor”, Journal of Bioscience and Bioengineering. 2017, 1-8. DOI: 10.1016/j.jbiosc.2017.07.014.
9. GIENTKA, I. et al. “Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glicerol”, Eur Food Res Technol. 2017, 243, 275-286. DOI: 10.1007/s00217-016-2742-9.
10. KONGRUANG, S., ROYTRAKUL, S. AND SRIARIYANUN, M. “Renewable biodiesel production from oleaginous yeast biomass using industrial wastes”,E3S Web of Conferences. 2020, 141. DOI: 10.1051/e3sconf/202014103010.
11. BOVIATSI, E. et al. “Valorisation of sugarcane molasses for the production of microbial lipids via fermentation of two Rhodosporidium strains for enzymatic synthesis of polyol esters”, J Chem Technol Biotechnol. 2019. DOI: 10.1002/jctb.5985.
12. SINGH, G. et al. “High density cultivation of oleaginous yeast isolates in ‘mandi’ waste for enhanced lipid production using sugarcane molasses as feed”, Fuel. 2020, 276. DOI: 10.1016/j.fuel.2020.118073.
13. SAGIA, S. et al. “Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw”, Electron. J. Biotechnol. 2020, 44. DOI: 10.1016/j.ejbt.2020.01.009.
14. GONZALEZ-GARCIA, Y. et al. “Lipids accumulation in Rhodotorula glutinis and Cryptococcus curvatus growing on distillery wastewater as culture medium”, Environ Prog Sustain Energy. 2013, 32, 69-74. DOI:10.1002/ep.10604.
15. EL-SALAM, B. A., AMER, A. E., AMER, M. E. “Using whey for production of carotenoids by Rhodotorulaglutinis”. Middle East Journal of Applied Sciences. 2014, 4(2), 385-391. ISSN 2077-4613.
16. SOMOGYI, M. “Notes on sugar determination”, J Biol. Chem. 1952. 159, 19-23. ISSN: 0021-9258.
17. LOWRY, O. H., ROSEBROUGH, N. J., et al., “Protein measurement with folin-phenol reagent”, J. Biol. Chem. 1951, 193, 265-275. ISSN: 0021-9258.
18. Clesceri, L.S., Greenberg, A. E., y Eaton, A. D. (Eds.). Standard Methods for the Examination of Water and Wastewater. 20a ed. Washington, D.C: American Public Health Association (APHA), American Water Works Association (AWWA), Water Environment Federation (WEF). 1998. ISBN 0-87553-235-7.
19. FRINGS, C. S., y DUNN, R. T. “A colorimetric method for determination of total serum lipids based on the sulfo-vanillin reaction”, Amer. J. Clin. Pathol. 1970, 53, 89. DOI: 10.1093/ajcp/53.1.89.
20. IZARD, J., y LIMBERGER, J. “Rapid screening method for quantization of bacterial cell lipids from whole cells, J Microbiol Methods. 2003, 355, 411–418. DOI: 10.1016/S0167-7012(03)00193-3.
21. OTERO, M. A. et al. “Composition and properties of sugarcane molasses from Northeastern Cuba”, Int. Sugar Journal. 1993, 95, 4-8. ISSN: 0020-8841.
22. AMORIM, H. V., BASSO, L. C., LOPES, M. L. Sugar cane juice and molasses, beet molasses and sweet sorghum: composition and usage. En: Ingledew, W. M.; Austin, G. D.; Kelsall, D. R., y Kluhspies, C. (Eds.). The alcohol textbook: a reference for the beverage, fuel, and industrial alcohol industries. 2da ed, Nottingham, Nottingham University Press, 2009, 39–46. ISBN: 9781904761655.
23. MOULIN, G.; GALZY, P. “Whey, a potential substrate for biotechnology”, Biotechnology and Genetic Engineering Reviews, 1984, 1 (1), 347-374. DOI:10.1080/02648725.1984.10647790.
24. JIRU, T. M. et al. “Production of single cell oil from cane molasses by Rhodotorula kratochvilovae (syn. Rhodosporidium kratochvilovae) SY89 as a biodiesel feedstock”, Chemistry Central Journal. 2018, 12, 91.DOI: 10.1186/s13065-018-0457-7.
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Published
2021-10-26
How to Cite
Serrat-Díaz, M., De la Fé-Isaac, Ángel D., & De la Fé-Isaac, J. A. (2021). Optimization of lipid production from whey and molasses by an oleaginous yeast strain. Revista Cubana De Química, 33(3), 274–290. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5186
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