Optimización de la producción de lípidos a partir de lactosuero y melaza por una cepa de levadura oleaginosa
Palabras clave:
biodiesel; lípidos; melaza; suero lácteo; subproductos agroindustriales.Resumen
Este trabajo tuvo como objetivo optimizar la producción de lípidos por una cepa de levadura oleaginosa, utilizando como fuente de carbono subproductos agroindustriales obtenidos de las industrias láctea y azucarera. Se utilizaron diseños de cribado (Plackett-Burman) y de optimización (Box-Behnken) para determinar la influencia de los componentes del medio de cultivo, sobre el porcentaje de lípidos acumulados y la concentración de biomasa obtenida, así como optimizar la composición del medio de cultivo, respectivamente. La concentración de triglicéridos se determinó por el método de la sulfo-fosfo-vainillina. Las ecuaciones de regresión obtenidas durante la optimización predicen valores máximos de acumulación de lípidos de 65 % y de concentración de biomasa de 13 mg/mL, resultados comparables a los obtenidos por otros investigadores y que apuntan hacia la aplicabilidad industrial de esta cepa en sistemas tecnológicos encaminados a la producción de biodiesel.
Citas
1. SCHNEIDER, T. et al. “Screening of industrial wastewaters as feedstock for the microbial production of oils for biodiesel production and high-quality pigments”, Journal of Combustion. 2012. ID 153410, 9 pag. DOI: 10.1155/2012/153410.
2. SCHNEIDER, T.et al. “Utilization of soluble starch by oleaginous red yeast Rhodotorula glutinis”, Journal of Sustainable Bioenergy Systems. 2013, 3, 57-63. DOI:10.4236/jsbs.2013.31007.
3. TAKAKU, H. et al. “Lipid metabolism of the oleaginous yeast Lipomyces starkeyi”, Applied Microbiology and Biotechnology. 2020, 104, 6141–6148. DOI: 10.1007/s00253-020-10695-9.
4. KAMINENI, A. and SHAW, J. “Engineering triacylglycerol production from sugars in oleaginous yeasts”, Current Opinion in Biotechnology. 2020, 62, 239–247. DOI: 10.1016/j.copbio.2019.12.022
5. AGEITOS, J. M. et al. “Oily yeasts as oleaginous cell factories”, Applied Microbiology and Biotechnology. 2011, 90 (4), 1219-1227. DOI: 10.1007/s00253-011-3200-z.
6. SAENGE, C. et al. “Efficient concomitant production of lipids and carotenoids by oleaginous red yeast Rhodotorula glutinis cultured in palm oil mill effluent and application of lipids for biodiesel production”, Biotechnology and Bioprocess Engineering. 2011, 16, 23-33. DOI: 10.1007/s12257-010-0083-2.
7. KITCHA, S.; CHEIRSILP, B. “Screening of oleaginous yeasts and optimization for lipid production using crude glycerol as a carbon source”, Energy Procedia. 2011, 9, 274-282, DOI: 10.1016/j.egypro.2011.09.029.
8. MANOWATTANA, A. et al. “Bioconversion of biodiesel-derived crude glycerol into lipids and carotenoids by an oleaginous red yeast Sporidiobolus pararoseus KM281507 in an airlift biorreactor”, Journal of Bioscience and Bioengineering. 2017, 1-8. DOI: 10.1016/j.jbiosc.2017.07.014.
9. GIENTKA, I. et al. “Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glicerol”, Eur Food Res Technol. 2017, 243, 275-286. DOI: 10.1007/s00217-016-2742-9.
10. KONGRUANG, S., ROYTRAKUL, S. AND SRIARIYANUN, M. “Renewable biodiesel production from oleaginous yeast biomass using industrial wastes”,E3S Web of Conferences. 2020, 141. DOI: 10.1051/e3sconf/202014103010.
11. BOVIATSI, E. et al. “Valorisation of sugarcane molasses for the production of microbial lipids via fermentation of two Rhodosporidium strains for enzymatic synthesis of polyol esters”, J Chem Technol Biotechnol. 2019. DOI: 10.1002/jctb.5985.
12. SINGH, G. et al. “High density cultivation of oleaginous yeast isolates in ‘mandi’ waste for enhanced lipid production using sugarcane molasses as feed”, Fuel. 2020, 276. DOI: 10.1016/j.fuel.2020.118073.
13. SAGIA, S. et al. “Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw”, Electron. J. Biotechnol. 2020, 44. DOI: 10.1016/j.ejbt.2020.01.009.
14. GONZALEZ-GARCIA, Y. et al. “Lipids accumulation in Rhodotorula glutinis and Cryptococcus curvatus growing on distillery wastewater as culture medium”, Environ Prog Sustain Energy. 2013, 32, 69-74. DOI:10.1002/ep.10604.
15. EL-SALAM, B. A., AMER, A. E., AMER, M. E. “Using whey for production of carotenoids by Rhodotorulaglutinis”. Middle East Journal of Applied Sciences. 2014, 4(2), 385-391. ISSN 2077-4613.
16. SOMOGYI, M. “Notes on sugar determination”, J Biol. Chem. 1952. 159, 19-23. ISSN: 0021-9258.
17. LOWRY, O. H., ROSEBROUGH, N. J., et al., “Protein measurement with folin-phenol reagent”, J. Biol. Chem. 1951, 193, 265-275. ISSN: 0021-9258.
18. Clesceri, L.S., Greenberg, A. E., y Eaton, A. D. (Eds.). Standard Methods for the Examination of Water and Wastewater. 20a ed. Washington, D.C: American Public Health Association (APHA), American Water Works Association (AWWA), Water Environment Federation (WEF). 1998. ISBN 0-87553-235-7.
19. FRINGS, C. S., y DUNN, R. T. “A colorimetric method for determination of total serum lipids based on the sulfo-vanillin reaction”, Amer. J. Clin. Pathol. 1970, 53, 89. DOI: 10.1093/ajcp/53.1.89.
20. IZARD, J., y LIMBERGER, J. “Rapid screening method for quantization of bacterial cell lipids from whole cells, J Microbiol Methods. 2003, 355, 411–418. DOI: 10.1016/S0167-7012(03)00193-3.
21. OTERO, M. A. et al. “Composition and properties of sugarcane molasses from Northeastern Cuba”, Int. Sugar Journal. 1993, 95, 4-8. ISSN: 0020-8841.
22. AMORIM, H. V., BASSO, L. C., LOPES, M. L. Sugar cane juice and molasses, beet molasses and sweet sorghum: composition and usage. En: Ingledew, W. M.; Austin, G. D.; Kelsall, D. R., y Kluhspies, C. (Eds.). The alcohol textbook: a reference for the beverage, fuel, and industrial alcohol industries. 2da ed, Nottingham, Nottingham University Press, 2009, 39–46. ISBN: 9781904761655.
23. MOULIN, G.; GALZY, P. “Whey, a potential substrate for biotechnology”, Biotechnology and Genetic Engineering Reviews, 1984, 1 (1), 347-374. DOI:10.1080/02648725.1984.10647790.
24. JIRU, T. M. et al. “Production of single cell oil from cane molasses by Rhodotorula kratochvilovae (syn. Rhodosporidium kratochvilovae) SY89 as a biodiesel feedstock”, Chemistry Central Journal. 2018, 12, 91.DOI: 10.1186/s13065-018-0457-7.
2. SCHNEIDER, T.et al. “Utilization of soluble starch by oleaginous red yeast Rhodotorula glutinis”, Journal of Sustainable Bioenergy Systems. 2013, 3, 57-63. DOI:10.4236/jsbs.2013.31007.
3. TAKAKU, H. et al. “Lipid metabolism of the oleaginous yeast Lipomyces starkeyi”, Applied Microbiology and Biotechnology. 2020, 104, 6141–6148. DOI: 10.1007/s00253-020-10695-9.
4. KAMINENI, A. and SHAW, J. “Engineering triacylglycerol production from sugars in oleaginous yeasts”, Current Opinion in Biotechnology. 2020, 62, 239–247. DOI: 10.1016/j.copbio.2019.12.022
5. AGEITOS, J. M. et al. “Oily yeasts as oleaginous cell factories”, Applied Microbiology and Biotechnology. 2011, 90 (4), 1219-1227. DOI: 10.1007/s00253-011-3200-z.
6. SAENGE, C. et al. “Efficient concomitant production of lipids and carotenoids by oleaginous red yeast Rhodotorula glutinis cultured in palm oil mill effluent and application of lipids for biodiesel production”, Biotechnology and Bioprocess Engineering. 2011, 16, 23-33. DOI: 10.1007/s12257-010-0083-2.
7. KITCHA, S.; CHEIRSILP, B. “Screening of oleaginous yeasts and optimization for lipid production using crude glycerol as a carbon source”, Energy Procedia. 2011, 9, 274-282, DOI: 10.1016/j.egypro.2011.09.029.
8. MANOWATTANA, A. et al. “Bioconversion of biodiesel-derived crude glycerol into lipids and carotenoids by an oleaginous red yeast Sporidiobolus pararoseus KM281507 in an airlift biorreactor”, Journal of Bioscience and Bioengineering. 2017, 1-8. DOI: 10.1016/j.jbiosc.2017.07.014.
9. GIENTKA, I. et al. “Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glicerol”, Eur Food Res Technol. 2017, 243, 275-286. DOI: 10.1007/s00217-016-2742-9.
10. KONGRUANG, S., ROYTRAKUL, S. AND SRIARIYANUN, M. “Renewable biodiesel production from oleaginous yeast biomass using industrial wastes”,E3S Web of Conferences. 2020, 141. DOI: 10.1051/e3sconf/202014103010.
11. BOVIATSI, E. et al. “Valorisation of sugarcane molasses for the production of microbial lipids via fermentation of two Rhodosporidium strains for enzymatic synthesis of polyol esters”, J Chem Technol Biotechnol. 2019. DOI: 10.1002/jctb.5985.
12. SINGH, G. et al. “High density cultivation of oleaginous yeast isolates in ‘mandi’ waste for enhanced lipid production using sugarcane molasses as feed”, Fuel. 2020, 276. DOI: 10.1016/j.fuel.2020.118073.
13. SAGIA, S. et al. “Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw”, Electron. J. Biotechnol. 2020, 44. DOI: 10.1016/j.ejbt.2020.01.009.
14. GONZALEZ-GARCIA, Y. et al. “Lipids accumulation in Rhodotorula glutinis and Cryptococcus curvatus growing on distillery wastewater as culture medium”, Environ Prog Sustain Energy. 2013, 32, 69-74. DOI:10.1002/ep.10604.
15. EL-SALAM, B. A., AMER, A. E., AMER, M. E. “Using whey for production of carotenoids by Rhodotorulaglutinis”. Middle East Journal of Applied Sciences. 2014, 4(2), 385-391. ISSN 2077-4613.
16. SOMOGYI, M. “Notes on sugar determination”, J Biol. Chem. 1952. 159, 19-23. ISSN: 0021-9258.
17. LOWRY, O. H., ROSEBROUGH, N. J., et al., “Protein measurement with folin-phenol reagent”, J. Biol. Chem. 1951, 193, 265-275. ISSN: 0021-9258.
18. Clesceri, L.S., Greenberg, A. E., y Eaton, A. D. (Eds.). Standard Methods for the Examination of Water and Wastewater. 20a ed. Washington, D.C: American Public Health Association (APHA), American Water Works Association (AWWA), Water Environment Federation (WEF). 1998. ISBN 0-87553-235-7.
19. FRINGS, C. S., y DUNN, R. T. “A colorimetric method for determination of total serum lipids based on the sulfo-vanillin reaction”, Amer. J. Clin. Pathol. 1970, 53, 89. DOI: 10.1093/ajcp/53.1.89.
20. IZARD, J., y LIMBERGER, J. “Rapid screening method for quantization of bacterial cell lipids from whole cells, J Microbiol Methods. 2003, 355, 411–418. DOI: 10.1016/S0167-7012(03)00193-3.
21. OTERO, M. A. et al. “Composition and properties of sugarcane molasses from Northeastern Cuba”, Int. Sugar Journal. 1993, 95, 4-8. ISSN: 0020-8841.
22. AMORIM, H. V., BASSO, L. C., LOPES, M. L. Sugar cane juice and molasses, beet molasses and sweet sorghum: composition and usage. En: Ingledew, W. M.; Austin, G. D.; Kelsall, D. R., y Kluhspies, C. (Eds.). The alcohol textbook: a reference for the beverage, fuel, and industrial alcohol industries. 2da ed, Nottingham, Nottingham University Press, 2009, 39–46. ISBN: 9781904761655.
23. MOULIN, G.; GALZY, P. “Whey, a potential substrate for biotechnology”, Biotechnology and Genetic Engineering Reviews, 1984, 1 (1), 347-374. DOI:10.1080/02648725.1984.10647790.
24. JIRU, T. M. et al. “Production of single cell oil from cane molasses by Rhodotorula kratochvilovae (syn. Rhodosporidium kratochvilovae) SY89 as a biodiesel feedstock”, Chemistry Central Journal. 2018, 12, 91.DOI: 10.1186/s13065-018-0457-7.
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2021-10-26
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Serrat-Díaz, M., De la Fé-Isaac, Ángel D., & De la Fé-Isaac, J. A. (2021). Optimización de la producción de lípidos a partir de lactosuero y melaza por una cepa de levadura oleaginosa. Revista Cubana De Química, 33(3), 274–290. Recuperado a partir de https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5186
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