Desarrollo de un sistema de partículas poliméricas nanoestructuradas para la liberación controlada del 5-fluorouracilo
Palavras-chave:
policianoacrilato de n-butilo; 5-Fluorouracilo; liberación controlada; partículas poliméricas.Resumo
Este trabajo describe el proceso de preparación de partículas poliméricas submicrométricas de policianoacrilato de n-butilo cargado con 5-fluorouracilo, diseñado como un sistema de liberación controlada para tratar carcinomas en piel, capaz de mejorar el valor terapéutico de este citostático, superando así las desventajas de su uso en formulaciones que lo contienen libre. El monómero de partida fue sintetizado y caracterizado mediante técnicas espectroscópicas. Las partículas se obtuvieron mediante polimerización en emulsión y se caracterizaron por FT-IR, RMN-1H, RMN-13C y MALDI-TOF. El tamaño de partícula, morfología y características electrocinéticas fueron analizados mediante DLS, SEM y potencial zeta. Dos metodologías fueron empleadas para cargar las partículas con el citostático: adsorción superficial y absorción o encapsulación en la matriz polimérica. Los procesos de carga fueron monitoreados empleando UV o HPLC. Las nanoesferas resultaron estables y de diámetro menor a 500nm. La eficiencia de encapsulación del 5-Fluorouracilo fue de 30% para ambas metodologías.
Referências
1. WAQAS, R.; SHAIKH, Z.; NAWAS, Y. “Epidemiology and Risk Factors of Basal Cell Carcinoma”. In: MIDGEN, M. R.; CHEN, L.; SILAPUNT S. (Eds.). Basal Cell Carcinoma. Cham: Springer Nature, 2020, pp 1-18. ISBN: 978-3-030-26887-9
2. OLIVEIRA, B., et al. “5-Fluorouracil, innovative drug delivery systems to enhance bioavailability for topical use”, Journal of Drug Delivery Science and Technology. 2020, 61 (102155), 1-11. ISSN: 1773-22473
3. PATEL, G.; DALWADI, C. “Cytotoxicity and Cellular Uptake of 5-Fluorouracil Loaded Methylcellulose Nanohydrogel for Treatment of Oral Cancer", Letters in Applied NanoBioScience. 2021, 10 (1), 1904-1918. ISSN: 2284-6808
4. MATUS-SANTOS, J. A., et.al “Revisión del monitoreo farmacocinético del 5-fluorouracilo como herramienta para incrementar eficacia y seguridad”, Revista Médica del Instituto Mexicano del Seguro Social. 2016, 54 (3), 354-362. ISSN: 0443-5117
5. CALIENNI, M., et al. “Nano-formulation for topical treatment of precancerous lesions: skin penetration, in vitro, and in vivo toxicological evaluation”, Drug Delivery and Translational Research. 2018, 8 (3), 496-514. ISSN: 2190-393X
6. MANSOORI, B., et al. “Hyaluronic acid‐ decorated liposomal nanoparticles for targeted delivery of 5‐ fluorouracil into HT‐ 29 colorectal cancer cells”, Journal of Cellular Physiology. 2020, 235 (10), 6817-6830. ISSN: 0021-9541
7. PELISSARI, F., et al. “Potential of nanoparticles as drug delivery system for cancer treatment”. In: INAMUDDIN, A. M.; ASIRI, A. M. (Eds.). Applications of Nanocomposite Materials in Drug Delivery. Oxford :Woodhead Publishing, 2018, pp 431-468. ISBN: 978-0-12813741-3
8. VAUTHIER, C.; LABARRE, D.; PONCHEL, G. “Design aspects of poly (alkylcyanoacrylate) nanoparticles for drug delivery”, Journal of Drug Targeting. 2007, 15 (10), 641-663. ISSN: 1061-186X
9. ARIAS, J. L., et al. “Study of carbonyl iron/poly (butylcyanoacrylate)(core/shell) particles as anticancer drug delivery systems: Loading and release properties”, European Journal of Pharmaceutical Sciences. 2008, 33 (3), 252-261. ISSN: 0928-0987
10. VAUTHIER, C., et al. “Poly (alkylcyanoacrylates) as biodegradable materials for biomedical applications”, Advanced Drug Delivery Reviews. 2003, 55 (4), 519-548. ISSN: 0169-409X
11. GAO, S., et al. "Polybutylcyanoacrylate nanocarriers as promising targeted drug delivery systems", Journal of Drug Targeting. 2015, 23 (6), 481-96. ISSN: 1061-186X
12. KATTAN, J., et al. “Phase I clinical trial and pharmacokinetic evaluation of doxorubicin carried by polyisohexylcyanoacrylate nanoparticles”, Investigational New Drugs. 1992, 10 (3), 191-199. ISSN: 0167-6997
13. ESTANQUEIRO, M., et al. “Nanotechnological carriers for cancer chemotherapy: the state of the art”, Colloids and surfaces B: Biointerfaces. 2015, 126, 631-48. ISSN: 0927-7765
14. OJHA, B., et al. “Nanotechnology: Introduction and Basic Concepts”. In: KUMAR, N.; JAIN, K. (Eds.). Dendrimers in Nanomedicine: Concept, Theory and Regulatory Perspectives. Boca Raton: CRC Press, 2021, pp.1-19. ISBN: 9778-1-003-02991-5
15. CHENG, H., et al. “Design of self-polymerized insulin loaded poly(n-butylcyanoacrylate) nanoparticles for tunable oral delivery”, Journal of Controlled Release. 2020, 321, 641-653. ISSN: 0168-3659
16. ARIAS, J. L., et al. “Magnetite/poly (alkylcyanoacrylate)(core/shell) nanoparticles as 5Fluorouracil delivery systems for active targeting”, European Journal of Pharmaceutics and Biopharmaceutics. 2008, 69 (1), 54-63. ISSN: 0939-6411
17. AHLIN, P., et al. “Investigation of polymeric nanoparticles as carriers of enalaprilat for oral administration”. International Journal of Pharmaceutics. 2002, 239 (1-2), 113-120. ISSN: 03785173
18. BENNET, D.; KIM, S. "Polymer Nanoparticles for Smart Drug Delivery". In: SEZER, A. D. (Eds.). Application of Nanotechnology in Drug Delivery. Rijeka: InTech, 2014, pp. 257-310. ISBN: 978-953-51-5756-4
19. ARIAS J. L., et al. “Poly (alkylcyanoacrylate) colloidal particles as vehicles for antitumour drug delivery: a comparative study”, Colloids and Surfaces B: Biointerfaces. 2008, 62 (1), 64-70. ISSN: 0927-7765
20. ARSALAN, A., et al. “Surface-modified polymeric nanoparticles for drug delivery to cancer cells”, Expert Opinion on Drug Delivery. 2021, 18 (1), 1-24. ISSN: 1742-5247
21. GRAF, A.; McDOWELL, A.; RADES, T. “Poly (alkycyanoacrylate) nanoparticles for enhanced delivery of therapeutics–is there real potential?”, Expert Opinion on Drug Delivery. 2009, 6 (4), 371-387. ISSN: 1742-5247
2. OLIVEIRA, B., et al. “5-Fluorouracil, innovative drug delivery systems to enhance bioavailability for topical use”, Journal of Drug Delivery Science and Technology. 2020, 61 (102155), 1-11. ISSN: 1773-22473
3. PATEL, G.; DALWADI, C. “Cytotoxicity and Cellular Uptake of 5-Fluorouracil Loaded Methylcellulose Nanohydrogel for Treatment of Oral Cancer", Letters in Applied NanoBioScience. 2021, 10 (1), 1904-1918. ISSN: 2284-6808
4. MATUS-SANTOS, J. A., et.al “Revisión del monitoreo farmacocinético del 5-fluorouracilo como herramienta para incrementar eficacia y seguridad”, Revista Médica del Instituto Mexicano del Seguro Social. 2016, 54 (3), 354-362. ISSN: 0443-5117
5. CALIENNI, M., et al. “Nano-formulation for topical treatment of precancerous lesions: skin penetration, in vitro, and in vivo toxicological evaluation”, Drug Delivery and Translational Research. 2018, 8 (3), 496-514. ISSN: 2190-393X
6. MANSOORI, B., et al. “Hyaluronic acid‐ decorated liposomal nanoparticles for targeted delivery of 5‐ fluorouracil into HT‐ 29 colorectal cancer cells”, Journal of Cellular Physiology. 2020, 235 (10), 6817-6830. ISSN: 0021-9541
7. PELISSARI, F., et al. “Potential of nanoparticles as drug delivery system for cancer treatment”. In: INAMUDDIN, A. M.; ASIRI, A. M. (Eds.). Applications of Nanocomposite Materials in Drug Delivery. Oxford :Woodhead Publishing, 2018, pp 431-468. ISBN: 978-0-12813741-3
8. VAUTHIER, C.; LABARRE, D.; PONCHEL, G. “Design aspects of poly (alkylcyanoacrylate) nanoparticles for drug delivery”, Journal of Drug Targeting. 2007, 15 (10), 641-663. ISSN: 1061-186X
9. ARIAS, J. L., et al. “Study of carbonyl iron/poly (butylcyanoacrylate)(core/shell) particles as anticancer drug delivery systems: Loading and release properties”, European Journal of Pharmaceutical Sciences. 2008, 33 (3), 252-261. ISSN: 0928-0987
10. VAUTHIER, C., et al. “Poly (alkylcyanoacrylates) as biodegradable materials for biomedical applications”, Advanced Drug Delivery Reviews. 2003, 55 (4), 519-548. ISSN: 0169-409X
11. GAO, S., et al. "Polybutylcyanoacrylate nanocarriers as promising targeted drug delivery systems", Journal of Drug Targeting. 2015, 23 (6), 481-96. ISSN: 1061-186X
12. KATTAN, J., et al. “Phase I clinical trial and pharmacokinetic evaluation of doxorubicin carried by polyisohexylcyanoacrylate nanoparticles”, Investigational New Drugs. 1992, 10 (3), 191-199. ISSN: 0167-6997
13. ESTANQUEIRO, M., et al. “Nanotechnological carriers for cancer chemotherapy: the state of the art”, Colloids and surfaces B: Biointerfaces. 2015, 126, 631-48. ISSN: 0927-7765
14. OJHA, B., et al. “Nanotechnology: Introduction and Basic Concepts”. In: KUMAR, N.; JAIN, K. (Eds.). Dendrimers in Nanomedicine: Concept, Theory and Regulatory Perspectives. Boca Raton: CRC Press, 2021, pp.1-19. ISBN: 9778-1-003-02991-5
15. CHENG, H., et al. “Design of self-polymerized insulin loaded poly(n-butylcyanoacrylate) nanoparticles for tunable oral delivery”, Journal of Controlled Release. 2020, 321, 641-653. ISSN: 0168-3659
16. ARIAS, J. L., et al. “Magnetite/poly (alkylcyanoacrylate)(core/shell) nanoparticles as 5Fluorouracil delivery systems for active targeting”, European Journal of Pharmaceutics and Biopharmaceutics. 2008, 69 (1), 54-63. ISSN: 0939-6411
17. AHLIN, P., et al. “Investigation of polymeric nanoparticles as carriers of enalaprilat for oral administration”. International Journal of Pharmaceutics. 2002, 239 (1-2), 113-120. ISSN: 03785173
18. BENNET, D.; KIM, S. "Polymer Nanoparticles for Smart Drug Delivery". In: SEZER, A. D. (Eds.). Application of Nanotechnology in Drug Delivery. Rijeka: InTech, 2014, pp. 257-310. ISBN: 978-953-51-5756-4
19. ARIAS J. L., et al. “Poly (alkylcyanoacrylate) colloidal particles as vehicles for antitumour drug delivery: a comparative study”, Colloids and Surfaces B: Biointerfaces. 2008, 62 (1), 64-70. ISSN: 0927-7765
20. ARSALAN, A., et al. “Surface-modified polymeric nanoparticles for drug delivery to cancer cells”, Expert Opinion on Drug Delivery. 2021, 18 (1), 1-24. ISSN: 1742-5247
21. GRAF, A.; McDOWELL, A.; RADES, T. “Poly (alkycyanoacrylate) nanoparticles for enhanced delivery of therapeutics–is there real potential?”, Expert Opinion on Drug Delivery. 2009, 6 (4), 371-387. ISSN: 1742-5247
Downloads
Publicado
2021-04-26
Como Citar
Ferrer-Viñals, D., Álvarez-Brito, R., & Baeza-Fonte, A. N. (2021). Desarrollo de un sistema de partículas poliméricas nanoestructuradas para la liberación controlada del 5-fluorouracilo. Revista Cubana De Química, 33(2), 113–135. Recuperado de https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5179
Edição
Seção
Artículos
Licença
Esta revista oferece acesso aberto imediato ao seu conteúdo, com base no princípio de que oferecer ao público o acesso gratuito à pesquisa contribui para uma maior troca global de conhecimento.
