Adaptation of a Colorimetric Micro Method for Quantification Soluble Phosphorus in Microbial Cultures

Authors

  • MSc. Odalys Rodríguez-Gámez Facultad de Ciencias Naturales. Universidad de Oriente, Santiago de Cuba
  • Lic. Isabel Aguilera-Rodríguez Facultad de Ciencias Naturales. Universidad de Oriente, Santiago de Cuba
  • Dra. C. Rosa María Pérez-Silva Facultad de Ciencias Naturales. Universidad de Oriente, Santiago de Cuba

Abstract

The present research focuses the adaptation of the colorimetric method of the ascorbic acid as micro method for the quantification of soluble phosphorus in microbial cultures, by determination of the parameters: linearity, precision, accuracy, detection and quantification limits. A solution of phosphate potassium dehydrogenate (KH2PO4) was used as reference intern pattern. The method can quantify soluble phosphorus values above 0,9 µg/mL. It was demonstrated that the micro method is exact, precise and lineal, for 95 % of confiability.
Key words: micro method, phosphate, solubilization, ascorbic acid.

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Published

2016-02-10

How to Cite

Rodríguez-Gámez, M. O., Aguilera-Rodríguez, L. I., & Pérez-Silva, D. C. R. M. (2016). Adaptation of a Colorimetric Micro Method for Quantification Soluble Phosphorus in Microbial Cultures. Revista Cubana De Química, 25(1), 3–8. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/803

Issue

Vol. 25 No. 1 (2013)

Section

Artículos

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