Verifying Validation of the Anthrone Colorimetric Method for Rhamnolipids Quantification

Authors

  • MSc. Odalys Rodríguez-Gámez Centro de Estudios de Biotecnología Industrial. Facultad de Ciencias Naturales. Universidad de Oriente, Santiago de Cuba
  • Javier E. Vilasó-Cadre Centro de Estudios de Biotecnología Industrial. Facultad de Ciencias Naturales. Universidad de Oriente, Santiago de Cuba
  • Lic. Isabel Aguilera-Rodríguez Centro de Estudios de Biotecnología Industrial. Facultad de Ciencias Naturales. Universidad de Oriente, Santiago de Cuba
  • Dra. Rosa M. Pérez-Silva Centro de Estudios de Biotecnología Industrial. Facultad de Ciencias Naturales. Universidad de Oriente, Santiago de Cuba
  • Dra. Arelis Ábalos-Rodríguez Centro de Estudios de Biotecnología Industrial. Facultad de Ciencias Naturales. Universidad de Oriente, Santiago de Cuba

Abstract

This paper aims to to validate by checking of anthrone colorimetric method to quantify rhamnolipids in microbial culture medium. Linearity, precision, accuracy, limits of detection and quantitation and robustness: This parameters were determined. It was demonstrated that the assay is linear and accurate in the range of 10-70 µg/mL of carbohydrates, coefficients lower than 3% variation for repeatability were obtained, and were found to have a detection limit equal to 1,63 mg / mL and a limit of quantification of 5,45 g / mL. It was found that the method is robust against variations in temperature and heating time as well as the amount of reagent added to the samples.
Keywords: validation, anthrone, rhamnolipid, colorimetric method

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Published

2016-01-07

How to Cite

Rodríguez-Gámez, M. O., Vilasó-Cadre, J. E., Aguilera-Rodríguez, L. I., Pérez-Silva, D. R. M., & Ábalos-Rodríguez, D. A. (2016). Verifying Validation of the Anthrone Colorimetric Method for Rhamnolipids Quantification. Revista Cubana De Química, 25(3), 287–294. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/617

Issue

Vol. 25 No. 3 (2013)

Section

Artículos

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