Microscale Validation of 4-Aminoantipyrine Test Method for Quantifying Phenolic Compounds in Microbial Cultures

Authors

  • Lic. Ibrahín Justiz-Mendoza Unidad Empresarial de Base de Elaboración de la Empresa Cervecera "Hatuey", Santiago de Cuba
  • Lic. Isabel Aguilera-Rodríguez Centro de Estudios de Biotecnología Industrial, Facultad de Ciencias Naturales, Universidad de Oriente, Santiago de Cuba
  • MSc. Irasema Pérez-Portuondo Centro de Estudios de Biotecnología Industrial, Facultad de Ciencias Naturales, Universidad de Oriente, Santiago de Cuba
  • Dra. C. Arelis Ábalos-Rodríguez Centro de Estudios de Biotecnología Industrial, Facultad de Ciencias Naturales, Universidad de Oriente, Santiago de Cuba
  • Dra. C. Rosa M. Pérez-Silva Centro de Estudios de Biotecnología Industrial, Facultad de Ciencias Naturales, Universidad de Oriente, Santiago de Cuba

Abstract

Validation of test methods microscale is currently of great importance due to the economic and environmental advantages possessed, which constitutes a prerequisite for the performance of services and quality assurance of the results to provide customer. This paper addresses the microscale validation of 4-aminoantipyrine spectrophotometric method for the quantification of phenolic compounds in culture medium. Parameters linearity, precision, regression, accuracy, detection limits, quantification limits and robustness were evaluated, addition to the comparison test with no standardized method for determining polyphenols (Folin Ciocalteu). The results showed that both methods are feasible for determining phenols.

Keywords: phenolic compounds, validation of analytical methods, 4-aminoantipyrine, culture medium.

Downloads

Published

2015-11-02

How to Cite

Justiz-Mendoza, L. I., Aguilera-Rodríguez, L. I., Pérez-Portuondo, M. I., Ábalos-Rodríguez, D. C. A., & Pérez-Silva, D. C. R. M. (2015). Microscale Validation of 4-Aminoantipyrine Test Method for Quantifying Phenolic Compounds in Microbial Cultures. Revista Cubana De Química, 26(2), 104–114. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/292

Issue

Vol. 26 No. 2 (2014)

Section

Artículos

License

This journal provides immediate open access to its content, based on the principle that offering the public free access to research helps a greater global exchange of knowledge. Each author is responsible for the content of each of their articles. 

Most read articles by the same author(s)