Comparison of methods using sulfuric acid for determination of total sugars

Authors

  • Xiomara López-Legarda Universidad de Antioquia, Medellín, Colombia
  • Andony Taramuel-Gallardo Universidad de Antioquia, Medellín, Colombia
  • Carolina Arboleda-Echavarría Universidad de Antioquia, Medellín, Colombia
  • Freimar Segura-Sánchez Universidad de Antioquia, Medellín, Colombia
  • Luis Fernando Restrepo-Betancur Universidad de Antioquia, Medellín, Colombia

Abstract

This article presents a comparison between phenol-sulfuric acid method and two variations, that propose to vary the order of reagents addition or remove phenol reaction. It was performed several calibration curves to compare the slopes obtained from the straight-line equation. It was confirmed that the addition of phenol is not necessary for the quantification of total carbohydrates. The ability of the method to quantify different sugars (glucose, xylose, galactose and 1,3-β-glucan), the stability of the reaction, the agitation and the reaction volume were evaluated. A validation of calibration curve was made. It was concluded that the method for the determination of total sugars does not require the use of phenol and can reduce the final reaction volume of 8 mL to 1,3 mL. This research has a positive impact in order to reduce costs of reagents and the amount of wastes.

Keywords: carbohydrates, sulfuric acid, phenol, spectrophotometry.

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Published

2017-06-09

How to Cite

López-Legarda, X., Taramuel-Gallardo, A., Arboleda-Echavarría, C., Segura-Sánchez, F., & Restrepo-Betancur, L. F. (2017). Comparison of methods using sulfuric acid for determination of total sugars. Revista Cubana De Química, 29(2), 180–198. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/2498

Issue

Vol. 29 No. 2 (2017)

Section

Artículos

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