Antimicrobial evaluation of the aqueous extract of the biomass of the microalga Phorphyridium cruentum

Authors

  • Dagmara Ferrer-Salas Departamento de Fundamentos Químicos y Biológicos, Facultad de Ingeniería Química y Agronomía, Universidad de Oriente, Cuba
  • Gabriel Llauradó-Maury Departamento de Farmacia, Facultad de Ciencias Naturales, Universidad de Oriente, Cuba
  • George Fernández-Duharte Laboratorio de Anticuerpos Monoclonales, Santiago de Cuba, Cuba
  • Yamila Lebeque-Pérez Centro de Estudios de Biotecnología, Universidad de Oriente, Cuba

Keywords:

Phorphyridium cruentum; antimicrobial activity; cytotoxic activity; tumor lines.

Abstract

Microalgae have proven to have multiple applications in the biotechnology industry. Porphyridium cruentum is a microalgae that exhibits a wide content of polysaccharides and proteins with potential pharmacological activity. This work evaluates the antimicrobial activity of the P. cruentum biomass against the parasites Leishmania infantum, Trypanosoma rhodesiense, Trypanosoma cruzi, and Trypanosoma brucei through the microdilution method in plates, as well as the cytotoxicity in leukocyte cell lines, using as a drug reference Tamoxifen. The biomass showed inhibitory activity against the parasites T. brucei, T. rhodesiensis, and T. cruzi. The study also demonstrated that the biomass does not exert cytotoxic activity on THP-1 human monocytes and RAW 264.7 murine macrophages. The results obtained indicate that the active compounds present in Porphyridium cruentum could be considered as possible antimicrobial candidates.

References

OLMEDO-GALARZA, V. “Carbohydrates and proteins in microalgaes: potential functional foods”. Brazilian Journal of Food Technology. 2019, 22. ISSN 1981-6723.

KHAN, M. I.; SHIN, J. H.; KIM, J. D. “The promising future of microalgae: current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products”. Microbial cell factories .2018, 17(1), 1-21. ISSN: 1475-2859.

DHANYA- SWATI, K. I; VANKA V. I.; K. S. & OSBORNE, W. J. “Antimicrobial activity of Ulvareticulata and its endophytes” Journal of Ocean University of China. 2016, 15(2), 363-369. ISSN: 1632-5182.

FALAISE, C., et al. “Antimicrobial Compounds from Eukaryotic Microalgae against Human Pathogens and Diseases in Aquaculture”. Marine Drugs. 2016, 14 (9), 159. ISSN: 1660-3397.

HAMIDI, M., et al. “Marine Bacteria versus Microalgae: Who Is the Best for Biotechnological Production of Bioactive Compounds with Antioxidant Properties and Other Biological Applications”. Marine Drugs. 2019 18(1), 28. ISSN: 1660-3397. 6. HUSSEIN, H. A. et al. “Phytochemical screening, metabolite profiling and enhanced antimicrobial activities of microalgal crude extracts in co-application with silver nanoparticle”. Bioresources and Bioprocessing, 2020. 7(1), 1-17. ISSN: 2197-4365.

SATHASIVAM, R.; RADHAKRISHNAN, R.; HASHEM, A.; ABD ALLAH, E. F. “Microalgae metabolites: A rich source for food and medicine”. Saudi Journal of Biological Sciences. 2019, 26(4), 709-722. ISSN: 1319-562X.

VERDUGO-GONZÁLEZ, L. et al. “Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsissp.” DYNA. 2019 86(211), pp. 192-198. ISSN: 0012-7361.

CANO-EUROPA, E. et al. “Uso terapéutico de algunos microorganismos, microalgas, algas y hongos”. Revista Mexicana de Ciencias Farmacéuticas. 2012, 43(4): 22-30. ISSN: 1870-0195.

VENKATESAN, R. et al. “Antibacterial activity of the marine diatom, Rhizosoleniaalata(Brightwell, 1858) against human pathogens”. Research Journal of Microbiology. 2007, 2(1): 98-100. ISSN: 1816-4935.

ABED, R. M. M.; DOBRETSOV, S.; SUDESH, K. “Applications of cyanobacteria in Biotechnology”. Journal of Applied Microbiology. 2009, 106(1): 1–12. ISSN: 1365-2672.

GONZÁLEZ-MENDOZA, J., MAGUIÑA-VARGAS, C., GONZÁLEZ-PONCE, F. M. “La resistencia a los antibióticos: un problema muy serio”. Acta Med. Peru. 2019; 36(2):145-51. ISSN 1728-5917.

ERBIL, G Ç.; ELP, M.; DURMAZ, Y. “Phycoerythrin Accumulation of Porphyridiumcruentum Culture at Indoor Tubular Photobioreactor”. University Journal of Agricultural Sciences. 2022, 32(1), 81-88. ISSN: 1308-7584.

BENAVIDES, M.; RITO-PALOMARES, M. “Simplified two-stage method to B-phycoerythrin recovery from Porphyridiumcruentum”. Journal of Chromatography B. 2006, 844(1): 39–44. ISSN 1570-0232.

NIIZAWA, Ignacio. "Diseño, modelado y optimización de foto-bioreactores destinados al cultivo de microalgas para diferentes aplicaciones tecnológicas". Tesis Doctoral. Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Argentina, 2015. http://hdl.handle.net/11185/878

BENAVIDES, J.; RITO–PALOMARES, M. “Bioprocess intensification: A potential aqueous two–phase process for the primary recovery of B–phycoerythrin from Porphyridiumcruentum.” J. Chromatogr. B. 2004; 807(1): 33–38. ISSN: 1570-0232. Doi: 10.1016/j.jchromb.2004.01.028

GABRIELSON, J. et al. “Evaluation of redox indicators and the use of digital scanners and spectrophotometer for quantification of microbial growth in microplates”. J. Microbiol Methods. 2002; 50(1): 63-73. ISSN: 1570-0232.

CHATELAIN, E. “Chagas Disease Drug Discovery: Toward a New Era”. Journal of Biomolecular Screening. 2015; 20(1) 22–35. ISSN:1308-7584. Doi: 10.1177/1087057114550585

CERVANTES-URIETA, V. A. et al. “Cultivo y composición bioquímica de diatomeas marinas (Bacillariophyta) de la Bahía de Santa Lucía, Acapulco, México”. Gayana. Botánica. 2020, 77(1), 11-22. ISSN: 0717-6643. Doi: 10.4067/S0717-66432020000100011

HERNÁNDEZ-MIRELES, T.; RITO-PALOMARES, M. “Improved recovery of B- phycoerythrin produced by the red microalga Porphyridiumcruentum”. Journal of 42 Chemical Technology & Biotechnology: International Research in Process, Environmental & Clean Technology. 2006; 81(6), 989-996. ISSN: 0268- 2575. Doi:10.1002/JCTB.1503

RITO-PALOMARES, M.; NÚNEZ, L.; AMADOR, D. “Aplicación práctica de sistemas bifásicos. Elementos para el desarrollo de un proceso prototipo para la recuperación de ficocianina Espirulinamaxima. “J. Chem Tecnología Biotecnología. 2001, 76(1), 1273-1280. ISSN: 1097-4660.

CASTRO-PIÑOL, M. et al. “Molecular docking of the interaction of 4, 5 - phenyl and 4, 5 - furylimidazoles with T. Cruci, T. Brucei and L. Infantum C y p 51 enzymes”. Rev. Cubana Quím. 2022, 34(1): 159–179. ISSN: 2224-5421.

HEMLATA, SUMBUL A., TASNEEM, F. “Extraction, purification and characterization of phycoerythrin from Michrochate and its biological activities”. Biocatalysis and Agricultural Biotechnology. 2018, 13(1), 84-89. ISSN: 1878-8181.

GÓMEZ-LUNA, L. et al. “Efecto de diferentes protocolos de aplicación de un campo magnético (0.03T) sobre el crecimiento, viabilidad y composición pigmentaria de Haematococcuspluvialis Flotow en suficiencia y ausencia de nitrógeno”. Biotecnología Vegetal. 2009, 9(2), 105 – 117. ISSN 2074-8647.

GUZMÁN-MURILLO, M.; ASCENCIO, F. “Antiadhesive activity of sulphathedexopolisaccharides of microalgae on attachmente of red sore disease associated bacteria and Heliobacter pylori to tissue culture cells”. Applied Micobiology. 2000; 12(6), 273-476. Doi: 10.1046/j.1472-765x.2000.00751.x

SÁNCHEZ-SAAVEDRA, M.; LICEA-NAVARRO, M.; BERNÁLDEZ-SARABIA, J. “Evaluation of the antibacterial activity of different species of phytoplankton”. Revista de Biología Marina y Oceanografía. 2010; 45(3), 531-536. ISSN: 0718-1957.

MORAES de J. et al. “Pinto, Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical disease Schistosomiasismansoni, PLoSNegl”. Trop. Dis. 2014, 8(1). doi.org/10.1371/journal.pntd.0002617

AllMENDINGER A. et al. “Antiprotozoal, antimycobacterial and cytotoxic potential of twenty-three British and Irish red algae”. Phytother. Res. 2010, 24(7), p.1099–1103. Doi.org/10.1002/ptr.3094

CHATELAIN, E. “Chagas disease drug Discovery”. J. Biomol. Screen. 2015, 20 (1)22–35. Doi.org/10.1177/1087057114550585

MONTIEL, G.; DÍAZ, G. “Respuesta inmune de las células del hospedero a la infección por Trypanosomacruzi”. Revista Médica del Hospital Nacional de Niños Dr. Carlos Sáenz Herrera. Costa Rica. 2002, 37(1):57-63. ISSN: 1017-8546.

Published

2023-01-17

How to Cite

Ferrer-Salas, D. ., Llauradó-Maury, G. ., Fernández-Duharte, G. ., & Lebeque-Pérez, Y. . (2023). Antimicrobial evaluation of the aqueous extract of the biomass of the microalga Phorphyridium cruentum. Revista Cubana De Química, 35(1), 88–104. Retrieved from https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5303

Issue

Section

Artículos

Most read articles by the same author(s)