Antioxidant activity of bioactive compounds isolated from leaves and bark of Gymnanthes lucida Sw
Keywords:
gymnanthes lucida Sw.; ellagitannins; coumarins; antioxidant activity.Abstract
Phytochemical investigations suggest that Gymnanthes lucida Sw. (aité) is a potential candidate for obtaining antioxidant principles. Total phenolic and flavonoid content were quantified and in vitro antioxidant capacity was assessed in extracts, ellagitannins, and coumarins isolated from the leaf and bark by three methods: 2,2-diphenyl-1-pricrylhydrazyl (DPPH) and 2,2´-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals scavenging activities and ferric reducing power. The highest phenolic and flavonoid content was observed in the leaf extract which also exhibited the highest activity among the extracts DPPH (IC50=12,82 ± 0,12 μg/mL), ABTS (IC50=13,83 ± 3,45 μg/mL), and reducing power (104% compared to ascorbic acid). Ellagic acid (EA) showed the highest activity, with DPPH (IC50=11,75 ± 0,53 μg/mL), ABTS (IC50=11,28 ± 0,28 μg/mL), and reducing power (107 % compared to ascorbic acid). Results suggest that G. lucida is a natural source of antioxidants where EA is the main secondary metabolite of the leaf responsible for the activity.
References
2. DÍAZ, J.; CELIA, C.; BISSE, J. “Estudio comparativo de Gymnanthes lucida Sw. en Cuba”. Revista del Jardín Botánico Nacional Universidad de La Habana. 1983, 4 (2), 19-36. ISSN 0253-5696
3. ROIG, J.T. Plantas Medicinales, Aromáticas o Venenosas de Cuba. 1st ed. Ciencia y Técnica, Editorial. La Habana: Cuba; 1974. ISBN 978-959-05-0809-7
4. OCHOA, A.P. et al. “Evaluación fitoquímica de hojas de Excoecaria lucida Sw.(Aité) (Euphorbiaceae) y Aislamiento e identificacion de un hemiterpenoide”. Revista Cubana de Plantas Medicinales. 2015, 20 (1), 117-130. ISSN 1028-4796
5. OCHOA, A.P. et al. “Bioassay-guided in vitro study of the antimicrobial and cytotoxic properties of the leaves from Excoecaria lucida Sw”. Pharmacognosy Research. 2017, 9 (4), 396-400. ISSN 0974-8490
6. OCHOA, A. Evaluación fitoquímica de hoja y corteza de Gymnanthes lucida Sw. (Yaití) (Euphorbiaceae). Doctoral thesis, Universidad de La Habana: Instituto De Farmacia Y Alimentos, La Habana, Cuba, 2017. Disponible en: http://eduniv.reduniv.edu.cu/index. php?page=13&id=26&db=1 Consultado: 15/03/2019
7. OCHOA, A. et al. Phytochemical Study of Nonpolar Extracts from Excoecaria lucida Sw. Leaves (Euphorbiaceae). Acta Chromatográfica. 2016, 28(3), 429–437. ISSN 2083-5736
APAK R, CAPANOGLU E, SHAHIDI F. Measurement of antioxidant activity and capacity: Recent trends and applications. 1st ed. Canada: Editorial John Wiley & Sons, 2018. ISBN 9781119135357
8. APAGÓN, S.M. Cumarinas en la prevención del estrés oxidativo dependiente de la edad. Doctoral thesis, Universidad Complutense de Madrid, Madrid, España, 1994. Disponible en: http://eprints.ucmes/id/eprint/3845/ Consultado: 20/06/2019 9. TUKIRAN, T.; WARDANA, A.P.; HIDAYATI, N.; & SHIMIZU, K. “An Ellagic Acid Derivative and Its Antioxidant Activity of Chloroform Extract of Stem Bark of Syzygium polycephalum Miq. (Myrtaceae)”. Indonesia Journal of Chemistry. 2018; 18 (1), 26-34. ISSN 1411-9420-2460-1578 10. FARNSWORTH, N.R. “Biological and Phytochemical Screening of Plants. International” Journal of Pharmaceutical Sciences. 1966; 55 (3), 262. ISSN 0975-1491.
12. KUMAZAWA, S.; HAMASAKA, T.; NAKAYAMA, T. “Antioxidant activity of propolis of various geographic origins”. Food Chemistry. 2004, 84 (3), 329-339. ISSN 0308-8146 13. PATRA, J.K. et al. “Screening of antioxidant and antifilarial activity of leaf extracts of Excoecaria agallocha L”. International Journal of Integrative Biology. 2009, 7 (1), 9-15. ISSN 0973-8363 14. KILIC, I.; YEŞILOĞLU, Y.; BAYRAK, Y. Spectroscopic studies on the antioxidant activity of ellagic acid. Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy. 2014, 130 (2014),447-52. ISSN 13861425
15. DEEPA, M.; DARSAN, M.B.; RAMALINGA, C. “In Vitro Evaluation of the antioxidant, anti-inflammatory and antiproliferative activities of the leaf extracts of Excoecaria agallocha L”. International Journal of Pharmaceutical Sciences. 2015, 7 (11), 346-52. ISSN 0975-1491 16. HOSSAIN, S. J.; AOSHIMA, H.; EL-SAYED, M.; & AHMED, F. Antioxidative and anti-histamine release activities of E. agallocha L. Pharmacologyonline. 2009, 2, 927-936. ISSN 1827-8620 17. SOFÍA, S. & TERESA, M.M. “Investigation of bioactive compounds and antioxidant activity of Excoecaria agallocha, L”. International Journal of Pharmaceutical Sciences Research. 2016, 7 (12), 5062-5066. ISSN 0975-8232
18. THIRUNAVUKKARASU, P. et al. “Screening of antioxidant status in selected mangrove plants in Pichvaram mangrove forest (south east coast of India). International Journal of Bioassays. 2013, 2 (3), 537-541. ISSN 2278-778X
19. MALIK, A. et al. In vitro antioxidant properties of Scopoletin. Journal of Chemistry and Pharmacy Research. 2011, 3 (3), 659-665. ISSN 0975-7384
20. DELLAGRECA, M. et al. Antioxidant and Radical Scavenging Properties of Malva sylvestris. Natural Products Communications. 2009, 4 (7), 893-896. ISSN 1555-9475
21. GIL, M.I. “Antioxidant Activity of Pomegranate Juice and Its Relationship with Phenolic Composition and Processing”. Journal of Agriculture Food Chemistry. 2000, 48(10), 4581-9. ISSN 1520-5118
22. KOSTOVA, I. et al. “Coumarins as Antioxidants”. Current Medicinal Chemistry. 2011, 18 (25), 3929-3951. ISSN 1875-533X
23. HOELZ, L.V.B. et al. “Quantitative structure-activity relationships of antioxidant phenolic compounds”. Journal of Chemical and Pharmaceutical Research. 2010, 2 (5), 291-306. ISSN 0975-7384
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