EVALUACIÓN DE LA VISCOSIDAD EN DISOLUCIONES DE HEMOGLOBINA HUMANA: UNA REVISIÓN
Palabras clave:
viscosidad; hemoglobina; glóbulos rojos; EPR; NMR.Resumen
RESUMEN
La viscosidad de la hemoglobina humana es un parámetro novedoso para evaluar clínicamente patologías que modifican la reología sanguínea. En este trabajo de revisión se analizan sus valores en disoluciones de concentración cercana a la intracelular y suspensiones de glóbulos rojos, así como los fundamentos teóricos y métodos experimentales utilizados en su determinación, para crear referencias útiles en el desarrollo de nuevas aplicaciones médicas o en el empleo de las existentes. Se incluyen determinaciones realizadas con viscosimetría (capilar, rotacional, “cuerpo
descendente”), resonancia paramagnética electrónica (RPE) y resonancia magnética nuclear (RMN). Los métodos más adecuados para evaluar la viscosidad en disoluciones de hemoglobina son la viscosimetría (6 mPa.s, 37 °C) y la relajación magnética nuclear (11 mPa.s, 20 °C). La RPE y los restantes métodos basados en RMN estiman valores de micro-viscosidad (1,5 mPa.s-2,0 mPa s) que pueden corregirse para obtener la viscosidad de la disolución.
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Derechos de autor 2025 Néstor J. Rodríguez-de la-Cruz, Claudia C. García-Cruz, Yamirka Alonso-Geli, Yulianela Mengana-Torres, Manuel A. Lores-Guevara
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