Estudio cinético de la producción de biomasa y compuestos fenólicos por Pleurotus ostreatus en fase sumergida
Abstract
En este trabajo se realizó el estudio cinético de la extracción de compuestos fenólicos a partir de la biomasa micelial de Pleurotus ostreatus,obtenida mediante fermentación sumergida. Se calcularon los factores de conversión de sustrato a biomasa (Yx/s), sustrato a producto (Yp/s)y la productividad de biomasa (Pxy Pp)correspondientes a: 0,39 g de biomasa·g de glucosa-1, 0,1 g de fenoles totales·g de glucosa-1, 0,047 g de biomasa L-1·h-1 y 0,0006 g de fenoles L-1·h-1, respectivamente. Se ajustaron los datos de crecimiento al modelo de Gompertz con un coeficiente de determinación (R2) de 0,88, mientras que los datos de consumo de glucosa se ajustaron mejor a un modelo Logístico con un valor de R2 igual a 0,95(p<0,05). Se obtuvieron los parámetros cinéticos tales como la velocidad específica de crecimiento (μ, 0,031 h-1) y la velocidad específica de consumo de glucosa (μg, 0,011 h-1).
Palabras clave: Pleurotus ostreatus, cultivo sumergido, compuestos fenólicos, biomasa.
References
MORRIS, H. J.; LLAURADÓ, G.; BELTRÁN, Y.; LEBEQUE, Y.“The Use of Mushrooms in the Development of Functional Foods, Drugs, and Nutraceuticals”. En: FERREIRA, I.; MORALES, P.; BARROS, L. (Eds.) Wild Plants, Mushrooms and Nuts: Functional Food Properties and Applications. Chichester, UK: John Wiley & Sons,2017, pp. 123-157.ISBN 9781118944639.
GREGORI, A.; ŠVAGELJ, M.; POHLEVEN, J. “Cultivation techniques and medicinal properties of Pleurotus spp”. Food Technology & Biotechnology.2007, 45 (3), 238-249. ISSN1330-9862
HORINCAR, V. B.; POPA, A. M.; PARFENE, G.; BAHRIM, G.“Evaluation of some biotechnological parameters influencing the Pleurotus ostreatus biomass production by submerged cultivation”. The Annals of the University of Dunarea de Jos of Galati. Fascicle VI. Food Technology.2015, 39 (2), 55-63. ISSN1843-5157
SLINKARD, K.; SINGLETON, V. L. “Total phenol analysis: automation and comparison with manual methods”. American Journal of Enology and Viticulture. 1977, 28(1), 49-55. ISSN0002-9254
MILLER, G. L. “Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar”. Analytical Chemistry.1959, 31(3), 426-428. ISSN0003-2700
FLICKINGER, M. C.; DREW, S.W. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation.1raedición. New York: John Wiley & Sons, 1999. ISBN 0-471-13822-3
WU, J.; CHEUNG, P. CK; WONG, K.; HUANG, N. “Studies on submerged fermentation of Pleurotus tuber-regium (Fr.)Singer—Part 1: physical and chemical factors affecting the rate of mycelial growth and bioconversion efficiency”. Food chemistry.2002, 81(2003), 389–393. ISSN 0308-8146
MCNEIL, B.; HARVEY, L. M. Practical Fermentatation Technology. London (UK): John Wiley & Sons, 2008. ISBN 978-0-470-01434-9.
PAPASPYRIDI, L.M.; KATAPODIS, P.; GONOU-ZAGOU, Z.; KAPSANAKIGOTSI, E. et al. “Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture”. Biochemical Engineering Journal.2010, 50(3), 131-138. ISSN 1369-703X
TANG, Y. J.; ZHU, L.W.; LI, H. M.“Submerged Culture of Mushrooms in Bioreactors –Challenges, Current State-of-the-Art, and Future Prospects”. Food Technology Biotechnology.2007, 45 (3), 221–229. ISSN 1330-9862
TEPWONG, P.; GIRI, A.; OHSHIMA, T. “Effect of mycelial morphology on ergothioneine production during liquid fermentation of Lentinula edodes”. Mycoscience.2012,53(2), 102–112. ISSN 1340-3540
DHILLON, G. S; BRAR, S. K.; KAUR, S. ; VERMA, M.“Rheological studies during submerged citric acid fermentation by Aspergillus niger in stirred fermentor using apple pomace ultrafiltration sludge”. Food and Bioprocess Technology.2013, 6(5), 1240-1250. ISSN 1935-5149
DORAN, P. M. "Chapter 10 - Mass Transfer." En: Bioprocess Engineering Principles (Second Edition). London: Academic Press, 2013, 379-444. ISBN 978-0-12-220851-5.
BIZUKOJC, M.; GONCIARZ, J. “Influence of oxygen on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 quantitatively studied on the level of individual pellets”. Bioprocess and Biosystems Engineering.2015, 38(7), 1251-1266. ISSN1615-7605
BIZUKOJC, M.; LEDAKOWICZ, S. "Bioprocess Engineering Aspects of the Cultivation of a Lovastatin Producer Aspergillus terreus." En: KRULL, R.; BLEY, T. eds. Filaments in Bioprocesses. Cham: Springer International Publishing, 2015, pp. 133-170. ISBN 978-3-319-20511-3.
KLAUS, A. M.; KOZARSKI, M.; NIKSIC, D.; JAKOVLJEVIC, N., et al. “Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune”. LWT-Food Sci. Technol.2011, 44(10), 2005-2011. ISSN 0023-6438
WASSER, S. P. “Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides”. Applied Microbiology and Biotechnology.2002, 60 (3), 258–274. ISSN1432-0614
WU, J.; CHEUNG, P. C.K.; WONG, K.; HUANG, N. “Studies on submerged fermentation of Pleurotus tuber-regium (Fr.) Singer. Part 2: effect of carbon-to-nitrogen ratio of the culture medium on the content and composition of the mycelial dietary fibre”. Food chemistry. 2003, 2004(85), 101-105. ISSN0308-8146
DÍAZ, R. "Efecto del pH inicial en el desarrollo de Pleurotus ostreatus en fermentación sumergida sobre su actividad de lacasas". Tesis de Maestría Instituto Politécnico Nacional, Tepetitla Tlax, México, 2009. Disponible en:https://www.repositoriodigital.ipn.mx/bitstream/123456789/6937/1/TESIS%20RUBEN%20DIAZ%20GODINEZ.pdf
MARQUEZROCHA, F. J; GUILLÉN, G. K.; SÁNCHEZ, J. E.; VAZQUEZDUHALT, R.“Growth characteristics of Pleurotus ostreatus in bioreactors”. Biotechnology techniques.1998, 13, 29-32.ISSN 0951-208X
ODUARDO, N. G. "Producción de setas comestibles y enzimas lacasas por fermentación en estado sólido de la pulpa de café con Pleurotus spp.". Tesis Doctoral Universidad de Oriente, Santiago de Cuba, 2008. Disponible en: www.imbe.fr/IMG/pdf/re-27_2008_doctorat_nora_garcia_oduardo.pdf
MARIANE, B.; NELSON,L.; GISELE, M. “Production of bioactive substances in fed-batch and semicontinuous culture by Pleurotus ostreatus”. Chemical Engineering Transactions.2014, 38, 307-312.ISSN 2283-9216
DE SOUZA, C. K.; FERRI, G. N.; TAVARES, L. B. B.; HERMANN, K. L. et al.“Evaluación de la influencia de los factores físicos y nutricionales en la propagación del micelio de Pleurotus sajor-caju CCB-019 en biomasa residual de embalaje de alimentos”. Revista do Congresso Sul Brasileiro de Engenharia de Alimentos.2015, 1(1), 1-7.ISSN 2447-3650
VAMANU, E. “Antioxidant properties of mushroom mycelia obtained by batch cultivation and tocopherol content affected by extraction procedures”. BioMed Research International. 2014, 2014, 1-8. ISSN 2314-6141
Downloads
Published
How to Cite
Issue
Section
License
This journal provides immediate open access to its content, based on the principle that offering the public free access to research helps a greater global exchange of knowledge. Each author is responsible for the content of each of their articles.