IN VITRO EVALUATION OF THE CYTOTOXICITY, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF THE SPENT Pleurotus MUSHROOM SUBSTRATE
Keywords:
animal health; Pleurotus ostreatus; spent substrate; antioxidant activity; antimicrobial activity.Abstract
Translator
The biological activities of the spent substrate from Pleurotus mushroom cultivated on coffee pulp have been little explored. This research evaluated the in vitro cytotoxicity, and the antioxidant/antimicrobial effects, of an aqueous extract (RTA) obtained from the spent substrate of P. ostreatus grown on coffee pulp in Cuba. Cytotoxicity was studied in the J774 cell line; total phenols/flavonoids, scavenging of DPPH radical and reducing power were evaluated for the antioxidant activity; and the antimicrobial assays by the disk diffusion method. No changes were found on cell viability. Total phenols and flavonoids were of 79,5 mg GAE/100 g and 48,6 mg QE/100 g, respectively, while the sequestration of DPPH radical was of 18,94 % and the reducing power of 0,11 absorbance units. RTA showed antimicrobial activity against Bacillus subtilis and Bacillus cereus. The results demonstrate the potentialities of Pleurotus spent substrate for applications as antioxidant/antimicrobial additive in animal food and health products.
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Copyright (c) 2025 Yaixa Beltrán-Delgado, Humberto J. Morris-Quevedo , Juan C. Ferrer-Romero, Onel Fong-Lores, David Garrido-Larramendi
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