Acoplamiento molecular de compuestos fenólicos de Pleurotus ostreatus con proteínas del balance redox
Abstract
Pleurotus sp es un género de hongos superiores, ampliamente distribuido a nivel mundial e incluye especies comestibles-medicinales de importancia económica. Dentro de este género se encuentra la especie Pleurotus ostreatus, la cual presenta diferentes propiedades biológicas con alto potencial para el tratamiento de enfermedades crónicas no trasmisibles que en la actualidad constituyen un serio problema de salud. En el presente trabajo se realiza la evaluación de la interacción entre los fenoles reportados para la especie Pleurotus ostreatus con proteínas del balance redox. A través de un docking molecular, con el programa Autodock y la base de datos de proteínas (PDB), se utilizaron trece fenoles y tres proteínas del balance redox (NFkB, NADPH –oxidasa y la COX-2), para visualizar el grado de afinidad entre estos y predecir sus mejores acoplamientos. Los resultaron mostraron valores adecuados de ∆G, Ki con las interacciones para la quercetina y catequina.
Palabras clave: fenoles, in silico, Pleurotus ostreatus, proteínas redox.
References
BARROS, I. et al. “Fatty acid and sugar compositions and nutritional value of five wild edible mushrooms from northeast Portugal”. Food Chem. 2007 a, 105, 140-5. ISSN:0308-8146.
DÍAZ, GODÍNEZ G. Fungal bioactive compounds: an overview. in : GV Kumar, MG Tuohy, O` Donovan A and M Lohani. Biotechonology of bioactive compounds: sources and applications. Chichester, West Sussex; Hoboken, NJ. USA : Wiley-Blackwell, 2015. ISBN:978-1-118-73349-3. 195-223 .
ZHANG, J. J. et al. “Bioactivities and Health Benefits of Mushrooms Mainly from China”. Molecules. 2016, 20, 21(7). pii: E938. doi: 10.3390/molecules21070938. ISSN: 1420-3049.
KUES, U.; LIU, Y. “Fruiting body production in basidiomycetes”, Appl Microbiol Biotechnol. 2000, 54, 141-152. ISSN: 1432-0614.
ZHONG, J. J.; XIAO, J. H. “Secondary metabolites from higher fungi: Discovery, bioactivity, and bioproduction”. Adv. Biochem. Eng. Biotechnol. 2009, 113, 7. ISSN: 0724-6145.
BORCHERS, A. T.; KEEN, C. L.; GERSTIWIN, M. E. “Mushrooms, tumors, and immunity: an update”. Exp. Biol. Med. 2004, 229, 393-406. ISSN: 1535-3702.
CHANG, S. T.; WASSER, S. “The role of culinary-medicinal mushrooms on human welfare with a pyramid model for human health”. Int J Med Mush. 2012, 14 (2), 95-134. ISSN: 1521-9437.
MORRIS, H. J. et al. “In vitro anti-proliferative effects on NB4 human leukemia cells and physicochemical screening of Pleurotus sp. (Higher Basidiomycetes). Mycelia from Cuba”. Int J Med Mus. 2014, 16 (3), 239 - 245. ISSN: 1521-9437.
COTE, J. et al. “Bioactive compounds in cranberries and their biological properties”, Critical Reviews in Food Science and Nutrition. 2010, 50, 666-679.ISSN:1040-8398.
D’ARCHIVIO, M. et al. “Bioavailability of the polyphenols: status and controversies”, International Journal of Molecular Sciences. 2010, 11, 1321-1342. ISSN:1422-007.
VALVERDE, M. E.; HERNANDEZ PEREZ, T.; PAREDES LOPEZ, O. “Edible mushrooms: improving human health and promoting quality life”. Int. J. Microbiol. 2015, 376387, 1–14. ISSN:1687-9198.
ABAGYAN, R; TOTROV, M. “High-throughput docking for lead generation”. Curr Opin Chem Biol. 2001, 5 (4), 375-82.ISSN:1367-5931.
PALACIOS, I. et al. “Antioxidant properties of phenolic compounds occurring in edible mushrooms”. Food Chem. 2011, 128, 674-678. ISSN:0308-8146.
WOLDEGIORGIS, A. Z.; ABATE, D; HAKI, G. D.; ZIEGLER, G. R. “Antioxidant property of edible mushrooms collected from Ethiopia”. Food Chem. 2014b, 157, 30-36. ISSN:0308-8146.
MUSZYNSKA, B.; SULKOWSKA ZIAJA, K.; EKIERT, H. “Phenolic acids in selected edible basidiomycota species: Armillaria mellea, Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus and Pleurotus ostreatus”. Acta Sci. Pol. Hortorum Cultus. 2013, 12, 107–116. ISSN:1644-0692.
GASECKA, M; MLECZEK, M; SIWULSKI, M; NIEDZIELSKI, P. “Phenolic composition and antioxidant properties of Pleurotus ostreatus and Pleurotus eryngii enriched with selenium and zinc”. Eur. Food Res. Technol. 2015, 226, 737–743. ISSN:1438-2385.
FERREIRA, I.; BARROS, L.; ABREU, R.M.V. “Antioxidants in wild mushrooms”, Curr. Med.Chem. 2009, 16, 1543–1560. ISSN:1875-533X.
BERMAN, H.M. et al. “The Protein Data Bank”, Nucleic Acids Research. 2000, 28, 235-242. ISSN:0305-1048.
FERRIN, T. et al. UCSF Chimera 2010, University of California, San Francisco.
TOMI, I.H.R. et al. “Synthesis, antimicrobial and docking study of three novel 2, 4, 5-triarylimidazole derivatives”, Journal of Saudi Chemical Society, 2013. ISSN:1319-6103.
DAVOOD, A; IMAN, M. “Molecular docking and QSAR study on imidazole derivatives as 14-demethylase inhibitors”. Turkish Journal of Chemistry. 2013, 37, 119-133. ISSN:1300-0527.
WALLACE, A.C; LASKOWSKI, R.A;THORNTON,J.M. “LIGPLOT: A program to generate schematic diagrams of proteinligand interactions”. Protein Eng. 1995, 127-134. ISSN:0269-2139.
DIMITRIOS, B. “Sources of natural phenolics antioxidants”. Trends in Food, Science and Technology. 2006, 17, 505-512.ISSN:0924-2244.
PETROVA, R.D. et al. “Fungal substances as modulators of NFKB activation pathway”. Mol Biol Rep. 2006, 34, 145-154.ISSN:1573-4978.
LEE, S.R; et al. “Renoprotective chemical constituents from an edible mushroom, Pleurotus cornucopiae in cisplatin-induced nephrotoxicity”. Bioorg. Chem. 2017, 71, 67–73. ISSN:0045-2068.
CHOI, D.Y.; LEE, Y.J.; HONG, J.T; LEE, H.J. “Antioxidant properties of natural polyphenols and their therapeutic potentials for Alzheimer’s disease”. Brain Res. Bull. 2012, 87, 144–153.ISSN:0361-9230.
SMITH, J. E.; ROWAN, N.J; SULLIVAN. R. “Medicinal mushrooms: their therapeutic properties and current medical usage with special emphasis on cancer treatments”. Cancer Research UK. University of Strathclyde, Glasgow, 2002.256 pages. B001A3XY4K. Deposited at the British Library. http://catalogue.bl.uk/F/?func=full-set-set&set_number=146966&set_entry=000001&format=999.
ALIA, M. et al. “Influence of quercetin and rutin on growth and antioxidant defense system of a human hepatoma cell line (HepG2)”. Eur J Nutr. 2006, 45, 19-28. ISSN:1436-6215.
MARUCA et al. “Chemoinformatic database building and in silico hit-identification of potential multi-targeting bioactive compounds extracted from mushroom species”, Molecules. 2017, 22, 1571; doi: 10.3390/molecules 22091571.ISSN: 1420-3049.
BIRT, D.F.; HENDRICH, S; WANG, W. “Dietary agents in cancer prevention: flavonoids and isoflavonoids”. Pharmacol Ther. 2001, 90, 157-177.ISSN:0163-7258.
RANELLETTI, F.O.; RICCI, R; LAROCCA L.M. “Growth-inhibitory effect of quercitin and presence of type-II estrogen binding sites in human colon-cancer cell lines and primary colorectal tumors”. Int J Cancer. 1992, 50, 486-492.ISSN:1097-0215.
YOSHIDA, M; SAKAI, T; HOSOKAWA, N. “The effects of quercitin on cell cycle progression and growth of human gastric cancer cells”. FEBS Lett. 1990, 260,10-13. ISSN:1873-3468.
FROUFE, H.J.C.; ABREU, R. M.V.; FERREIRA, I. C.F.R. “Using molecular docking to investigate the anti-breast cancer activity of low molecular weight compounds present on wild mushrooms”. SAR and QSAR in Environmental Research. 2011, 22 (3-4), 315-328. DOI: 10.1080/1062936X.2011.569897. ISSN: 1062-936X.
Downloads
Published
How to Cite
Issue
Section
License
This journal provides immediate open access to its content, based on the principle that offering the public free access to research helps a greater global exchange of knowledge. Each author is responsible for the content of each of their articles.
