Estabilidad físico-química del extracto hidroetanólico al 70 % de hojas de Spondias mombin L
Palavras-chave:
spondias mombin L.; anacardiaceae; plantas medicinales; estabilidad.Resumo
El objetivo del presente trabajo fue evaluar la estabilidad a temperatura ambiente, por un periodo de un año, del extracto en etanol al 70 % de las hojas de la especie Spondias mombin L. Esta planta se usa para el tratamiento de diversas enfermedades, debido principalmente a su actividad antibacteriana, antiinflamatoria y antioxidante. El material vegetal fue recolectado en diferentes etapas del ciclo de vida y se utilizó la maceración como método de extracción. El análisis químico por cromatografía líquida de alta resolución mostró la presencia mayoritaria de ácido gálico y derivados y de la quercetina 3-O-glicosilada. Fueron determinados los siguientes parámetros: características organolépticas, pH, densidad relativa, sólidos totales, sólidos fijos, sólidos volátiles, contenido total de fenoles y contenido total de flavonoides; se establecieron además los intervalos de confianza. Los datos obtenidos del extracto sometido al estudio de estabilidad, relacionados con estos parámetros, permiten asegurar comportamiento estable durante un año, desde un punto de vista físico-químico.
Referências
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20. A. O. AYOKA, et al. “Sedative, antiepileptic and antipsychotic effects of Spondias mombin L. (Anacardiaceae) in mice and rats”, Journal of Ethnopharmacology. 2006, 103 (2), 166–175. DOI 10.1016/j.jep.2005.07.019.
21. MINSAP. NRSP No. 309. Medicamentos de origen vegetal: droga cruda. Métodos de ensayos. La Habana: MINSAP, 1992. https://cecmed.cu.
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25. ENGELS, C. et al. “Characterization of phenolic compounds in jocote (Spondias purpurea L.) peels by ultra-high-performance liquid chromatography/electrospray ionization mass spectrometry”, Food Research International. 2012, 46, 557–562. doi:10.1016/j.foodres.2011.04.003.
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29. LAKENBRINK, C., et al. “Flavonoids and other polyphenols in consumer brews of tea and other caffeinated beverages”. Journal of Agricultural and Food Chemistry. 2000, 48, 2848–2852. https://doi.org/10.1021/jf9908042.
30. SIMIC´, A. Z. et al. “Study of ellagic acid electro-oxidation mechanism”. Monatshefte fur Chemie. 144, 121-128, 2013. DOI 10.1007/s00706-012-0856-8.
31. ROMANI, A.; CAMPO, M. and PINELLI, P. “HPLC/DAD/ESI-MS analyses and anti-radical activity of hydrolyzable tannins from different vegetal species”. Food Chemistry. 2012, 130, 214-221. doi: 10.1016/j.foodchem.2011.07.009.
32. DOURADO, R. S. y LADEIRA, A, M. “Identificação de flavonóides em Hypericum cordatum (Vell.) N. Robson (Clusiaceae)”. Brazilian Journal of Botany. 2008, 31(4), 611-620. ISSN 1806-9959. http://dx.doi.org/10.1590/S0100-84042008000400007.
33. AVALOS-GARCÍA, A. y PÉREZ-URRIA CARRIL. “Metabolismo secundario de plantas”. Reduca (Biología). Serie Fisiológica Vegetal. 2009, 2(3), 119-145. ISSN: 189-3620.
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35. MITCHELL, J. D. y DALY D. C. “A revision of Spondias L. (Anacardiaceae) in the Neotropics”. PhytoKeys. 2015, 55, 1–92. doi: 10.3897/phytokeys.55.8489.
36. SOKOLOVÁ, R. et al. “The oxidation of natural flavonoid quercetin”, Chemical Communications. 2012, 48, 3433–3435. DOI: 10.1039/c2cc18018a.
2. LÓPEZ-REGUEIRO, S.; et al. “Fitoterapia como coadyuvante en el tratamiento de la obesidad”, Farmacéuticos Comunitarios. 2012, 4(4), 166-174. ISSN: 2173-9218.
3. GAFNER, S. and BERGUERON, C. “The challenges of chemical stability testing of herbal extract in finished products using State-of-the-Art analytical methodologies”. Current Pharmaceutical Analysis. 2005, 1, 203-215. DOI 10.2174/157341205402.
4. OSUNTOKUN, O. T. “Exploring the Medicinal Efficacy, Properties and Therapeutic uses of Spondias mombin (Linn)”, International Journal of Applied Research on Medicinal Plants. 2019, 2, 115-123. DOI: 10.29011/IJARMP-115.100115.
5. AKOYA, A. O., et al. “Medicinal and economic value of Spondias mombin”, African Journal of Biomedical Research. 2008, 11, 129 - 136. DOI 10.4314/ajbr.v11i2.50714.
6. SAMEH, S.; et al. “Genus Spondias: A Phytochemical and Pharmacological Review”, Evidence-Based Complementary and Alternative Medicine. 2018, Article ID 5382904, 13 pages. https://doi.org/10.1155/2018/5382904.
7. PÉREZ-PORTERO, Y. et al. “Plantas antidermatofíticas, utilizadas en comunidades costeras del municipio Guamá, Santiago de Cuba”, Etnobiología. 2009, 7, 56-62. ISSNe 2448-8151. ISSN 1665-2703.
8. ARAÚJO DA SILVA, A. R. et al. “Chemical composition, antioxidant and antibacterial activities of two Spondias species from North-eastern Brazil”, Pharmaceutical Biology. 2012, 50 (6), 740-746. DOI: 10.3109/13880209.2011. 627347.
9. ALVES-BRITO, S. et al. “Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.” Hindawi Oxidative Medicine and Cellular Longevity, 2018, ID 1731459, 20 pages. https://doi.org/10.1155/2018/1731459.
10. CORTHOUT, J. “Antiviral ellagitannins from Spondias mombin”, Phytochemistry. 1991, 30(4), 1129-1130. https://doi.org/10.1016/S0031-9422(00)95187-2.
11. SILVA, A. R. A., et al. “Antiviral activities of extracts and phenolic components of two Spondias species against dengue virus”, The Journal of Venomous Animals and Toxins including Tropical Diseases. 2011, 17, 406–413. ISSN 1678-9199.
12. FRED-JAIYESIMI, A.; KIO, A. y RICHARD, W. “α-Amylase inhibitory effect of 3b-olean-12-en-3-yl (9Z)-hexadec-9-enoate isolated from Spondias mombin leaf”, Food Chemistry. 2009, 116, 285–288. doi:10.1016/j.foodchem.2009.02.047.
13. CABRAL, B. et al. “Phytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves”, Revista Brasileira de Farmacognosia. 2016, 26, 304–311. http://dx.doi.org/10.1016/j.bjp.2016.02.002.
14. REY-BLANES, C. et al. “In vitro evaluation of the antitumoral and antiangiogenic effects of extracts from Spondias mombin L. leaves”, Biomedicine & Pharmacotherapy.2020, 131, 110716. https://doi.org/10.1016/j.biopha.2020. 110716.
15. CHUKWUKA, N.; UCHENDU, A. AND ISEK, T. “Antifertility activity of aqueous ethanolic leaf extract of Spondias mombin (Anacardiaceae) in rats”, Health Sciences, 2008, 8(3), 163-167. PMCID: PMC2583269. PMID: 19357744. ISSN 2372-5079
16. NKANU, E. E. “In vivo hypolipidemic and hypoglycemic effects of aqueous extract of Spondias mombin leaves and detoxification of reactive oxygen species in alloxan-induced diabetic rats”, International Journal of Biological Chemistry Science. 2016, 10(4), 1573-1579. ISSNe 1997-342X ISSN 1991-8631.
17. IGWE, C.U. et al. “Evaluation of the Chemical Compositions of the Leaf of Spondias Mombin Linn from Nigeria”, Australian Journal of Basic and Applied Sciences. 2010, 4(5), 706-710. ISSN 1991-8178.
18. PEREIRA, C. et al. “Phytochemical and phylogenetic Analysis of Spondias (Anacardiaceae)”, Química Nova. 2015, 38(6), 813-816. http://dx.doi.org/10.5935/0100-4042.20150087.
19. PROCHÁZKOVÁ, D.; BOUŠOVÁ I.; WILHELMOVÁ, N. “Antioxidant and prooxidant properties of flavonoids”, Fitoterapia. 2011, 82, 513–523. http://doi:10.1016/j.fitote.2011.01.018.
20. A. O. AYOKA, et al. “Sedative, antiepileptic and antipsychotic effects of Spondias mombin L. (Anacardiaceae) in mice and rats”, Journal of Ethnopharmacology. 2006, 103 (2), 166–175. DOI 10.1016/j.jep.2005.07.019.
21. MINSAP. NRSP No. 309. Medicamentos de origen vegetal: droga cruda. Métodos de ensayos. La Habana: MINSAP, 1992. https://cecmed.cu.
22. MINSAP. NRSP No. 311. Medicamentos de origen vegetal: Extractos fluidos y tinturas. Procesos tecnológicos. La Habana: MINSAP, 1992. https://cecmed.cu.
23. HARBORNE J. B. Phytochemical Methods. 3ed. London: Chapman - Hall, 1998. ISBN-13: 978-0-412-23050-9 e-ISBN-13: 978-94-009-5921-7. DOl: 10.1007/978-94-009-5921-7
24. PÉREZ-PORTERO, Y. et al. “Caracterización fitoquímica de extractos de Spondias mombin L. (Anacardiaceae)”, Revista Cubana de Química. 2013, 25(2), 150-153. ISSNe 2224-5421.
25. ENGELS, C. et al. “Characterization of phenolic compounds in jocote (Spondias purpurea L.) peels by ultra-high-performance liquid chromatography/electrospray ionization mass spectrometry”, Food Research International. 2012, 46, 557–562. doi:10.1016/j.foodres.2011.04.003.
26. MINSAP. NRSP No. 312. Métodos de ensayos para la determinación de los requisitos de los extractos fluidos y tinturas. La Habana: MINSAP, 1992. https://cecmed.cu.
27. APHA. Standard Methods for the Examination of Water and Wastewater. 2540 B, 2540E Edition 20. Washington: APHA/AWWA/WEF, 1998. ISBN 0-87553-235-7.
28. SINGLETON, V. L., ORTHOFER, R. y LAMUELA-RAVENTOS, R. M. “Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent”. Methods Enzymology. 1999, 299, 152-78. https://doi.org/10.1016/S0076-6879(99)99017-1.
29. LAKENBRINK, C., et al. “Flavonoids and other polyphenols in consumer brews of tea and other caffeinated beverages”. Journal of Agricultural and Food Chemistry. 2000, 48, 2848–2852. https://doi.org/10.1021/jf9908042.
30. SIMIC´, A. Z. et al. “Study of ellagic acid electro-oxidation mechanism”. Monatshefte fur Chemie. 144, 121-128, 2013. DOI 10.1007/s00706-012-0856-8.
31. ROMANI, A.; CAMPO, M. and PINELLI, P. “HPLC/DAD/ESI-MS analyses and anti-radical activity of hydrolyzable tannins from different vegetal species”. Food Chemistry. 2012, 130, 214-221. doi: 10.1016/j.foodchem.2011.07.009.
32. DOURADO, R. S. y LADEIRA, A, M. “Identificação de flavonóides em Hypericum cordatum (Vell.) N. Robson (Clusiaceae)”. Brazilian Journal of Botany. 2008, 31(4), 611-620. ISSN 1806-9959. http://dx.doi.org/10.1590/S0100-84042008000400007.
33. AVALOS-GARCÍA, A. y PÉREZ-URRIA CARRIL. “Metabolismo secundario de plantas”. Reduca (Biología). Serie Fisiológica Vegetal. 2009, 2(3), 119-145. ISSN: 189-3620.
34. CRISTOFOLI, N. L. et al. “Antioxidant and antimicrobial potential of cajazeira leaves (Spondias mombin) extracts”. Separation Science and Technology. 2018, 54(4), 1-11 ISSN: 0149-6395 (Print) 1520-5754 (Online). https://doi.org/10.1080/01496395.2018.1508233.
35. MITCHELL, J. D. y DALY D. C. “A revision of Spondias L. (Anacardiaceae) in the Neotropics”. PhytoKeys. 2015, 55, 1–92. doi: 10.3897/phytokeys.55.8489.
36. SOKOLOVÁ, R. et al. “The oxidation of natural flavonoid quercetin”, Chemical Communications. 2012, 48, 3433–3435. DOI: 10.1039/c2cc18018a.
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2021-10-26
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Pérez-Portero, Y., Vera-Ayala, A., García-Díaz, J., Escalona-Arranz, J. C., & Casals-Hung, M. (2021). Estabilidad físico-química del extracto hidroetanólico al 70 % de hojas de Spondias mombin L. Revista Cubana De Química, 33(3), 305–325. Recuperado de https://cubanaquimica.uo.edu.cu/index.php/cq/article/view/5188
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